PXD075247-1

PXD075247 is an original dataset announced via ProteomeXchange.

Title	Competitive kinobead profiling of RAF inhibitors
Description	Kinase enrichment was performed using kinobeads followed by LC–MS/MS to profile target engagement/competition by RAF inhibitors. HEK293T lysates overexpressing 185 protein kinases were prepared in modified RIPA buffer, pre-incubated with DMSO or inhibitor, then incubated with kinobeads to enrich kinases. Beads were washed, proteins denatured, and on-bead digestion was performed (Lys-C/Trypsin). Resulting peptides were cleaned up and analyzed by LC–MS/MS.
HostingRepository	PRIDE
AnnounceDate	2026-04-20
AnnouncementXML	Submission_2026-04-20_08:35:31.131.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Brush
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-04 19:30:44	ID requested
⏵ 1	2026-04-20 08:35:31	announced

10.1038/S41589-026-02212-2;

submitter keyword: Human, LC-MS, Kinase

Dustin James Maly
contact affiliation	Department of Chemistry, University of Washington
contact email	djmaly@uw.edu
lab head
Daniel Brush
contact affiliation	University of Washington
contact email	dbrush@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075247

PRIDE project URI

• PRIDE
  1. PXD075247
     1. Label: PRIDE project
     2. Name: Competitive kinobead profiling of RAF inhibitors