PXD075184-1

PXD075184 is an original dataset announced via ProteomeXchange.

Title	Affinity purification–mass spectrometry of TaRIPK-associated fungal proteins
Description	OE-TaRIPK-#2 plants were inoculated with FGJQ. To enhance fungal infection efficiency, an additional set of OE-TaRIPK-#2 plants was treated with BSMV:TaRBOHD prior to FHGQ inoculation. At 9 dpi, total proteins were extracted from each group using radio immunoprecipitation assay (RIPA) buffer and desalted using Zeba™ spin desalting columns. The purified proteins were incubated with 30 μL myc-conjugated agarose beads in binding buffer at 4°C overnight under gentle rotation. After centrifugation at 2,000 x g for 3 min at 4°C, the pellet was washed sequentially with Buffer I (2% sodium dodecyl sulfate (SDS), Buffer II (50 mM HEPES, 500 mM NaCl, 1 mM EDTA, 0.1% deoxycholic acid, and 1% Triton X-100), and Buffer III (10 mM Tris–HCl, 250 mM LiCl, 1 mM EDTA, 0.1% deoxycholic acid, and 1% IGEPAL® CA-630. Following six washes with 50 mM ammonium bicarbonate, proteins were digested with trypsin (1:50) at 37°C overnight.
HostingRepository	iProX
AnnounceDate	2026-03-04
AnnouncementXML	Submission_2026-03-03_23:09:24.150.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinhai Wang
SpeciesList	scientific name: Triticum aestivum; NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-03 23:09:07	ID requested
⏵ 1	2026-03-03 23:09:24	announced

Dataset with its publication pending

submitter keyword: Wheat, leaf rust, TaRIPK, effector

Zaifeng Li
contact affiliation	Hebei Agricultural University
contact email	lzf7551@aliyun.com
lab head
Xinhai Wang
contact affiliation	Hebei Agricultural University
contact email	wangxinhai1992@163.com
dataset submitter

iProX dataset URI