PXD075181-1

PXD075181 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic profiles of early-stage changes in LPS-induced macrophage polarization
Description	This study integrated proteomic and phosphoproteomic analyses to systematically delineate the early-stage (0–6 hours) dynamic molecular landscape of lipopolysaccharide (LPS)-induced polarization of murine RAW264.7 macrophages toward the M1 phenotype. The results revealed that both differentially expressed proteins and phosphorylation sites exhibited a distinct two-stage change pattern during early polarization: proteins in Stage I (adaptive regulation stage) were primarily involved in functional groundwork preparation such as inflammatory priming and cell cycle regulation, whereas the persistently up-regulated proteins in Stage II (delayed response stage) were significantly enriched in pathways including NF-κB signaling and interferon response, driving core anti infection and pro-inflammatory functions. Notably, alterations in protein phosphorylation occurred earlier and more rapidly than changes in protein expression, indicating the leading role of post translational modifications in initiating the polarization process. Kinase prediction analysis further identified key kinase families such as CDK and AKT in regulating early phosphorylation events. These findings not only elucidate the dynamic regulatory network during the early phase of macrophage M1 polarization, but also provide potential molecular targets and a theoretical basis for precisely timed intervention in inflammation-related diseases such as autoimmune disorders, infectious diseases, and inflammatory bone diseases.
HostingRepository	iProX
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-03_19:46:38.902.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiyu Wu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-03 19:42:01	ID requested
⏵ 1	2026-03-03 19:46:39	announced

Dataset with its publication pending

submitter keyword: macrophage polarization, proteome, phosphoproteome, stage-specific changes, inflammation

Manjiangcuo Wang
contact affiliation	West China Hospital, Sichuan University
contact email	18811594622@163.com
lab head
Xiyu Wu
contact affiliation	West China Hospital, Sichuan University
contact email	wuxiyu@wchscu.cn
dataset submitter

iProX dataset URI