PXD075147 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PCP-MS analysis of insulin resistance in C2C12 myotubes with or without acute insulin stimulation |
| Description | To study dynamic remodelling of protein complexes, C2C12 myotubes were treated with 0.5 mM palmitic acid for 24 h to induce insulin resistance (IR) and then stimulated with or without acute 100 nM insulin for 30 min followed by PCP-MS(n=3 each. Samples were lysed in non-denaturing buffer, separated by BN-PAGE, and each lane cut into 30 x 1.5mm fractions. Each fraction underwent in-gel digestion and was analysed with liquid chromatography – tandem mass spectrometry (LC-MS/MS) using data-independent acquisition (DIA) resulting in the analysis of 360 individual runs. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-29 |
| AnnouncementXML | Submission_2026-05-28_18:17:44.618.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Benjamin Parker |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-03 08:22:11 | ID requested | |
| ⏵ 1 | 2026-05-28 18:17:45 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: protein correlation profiling, C2C12,insulin resistance |
Contact List
| Benjamin Parker |
|---|
| contact affiliation | The University of Melbourne |
| contact email | ben.parker@unimelb.edu.au |
| lab head | |
| Benjamin Parker |
|---|
| contact affiliation | The University of Melbourne |
| contact email | ben.parker@unimelb.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075147 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075147
- Label: PRIDE project
- Name: PCP-MS analysis of insulin resistance in C2C12 myotubes with or without acute insulin stimulation
