PXD075115-1

PXD075115 is an original dataset announced via ProteomeXchange.

Title	NDUFV1 Activation Mediates High Glucose-Induced Apoptosis in Peritoneal Mesothelial Cells
Description	To elucidate the mechanisms underlying high glucose-induced Peritoneal mesothelial cells (PMCs) apoptosis, we obtained the first dynamic proteomic atlas of HMrSV5 cells under different concentration of glucose and mannitol stimulation. After excluding osmosis, 626 differentially expressed proteins (DEPs) were detected following high glucose stimulation. Notably, the up-regulated DEPs were significantly enriched in the oxidative phosphorylation pathway, implicating mitochondrial dysfunction in the apoptotic process. Protein-protein interaction network analysis further revealed that NDUFV1, a core subunit of mitochondrial Complex I, might be a key regulator. To validate clinical relevance, we performed a comparative proteomic analysis of peritoneal dialysis (PD) effluents from new and long-term PD patients. The clinical proteomics data revealed a marked enrichment of oxidative phosphorylation in the early-stage of dialysis patients, coinciding with notably up-regulation of NDUFV1. Molecular biology experiments demonstrated that the up-regulation of NDUFV1 increased reactive oxygen species production, disrupted mitochondrial membrane potential, thereby promoting apoptosis.
HostingRepository	iProX
AnnounceDate	2026-03-03
AnnouncementXML	Submission_2026-07-16_23:58:14.796.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chen Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-02 17:12:45	ID requested
⏵ 1	2026-07-16 23:58:15	announced

Dataset with its publication pending

submitter keyword: Peritoneal dialysis, Proteomics, High glucose, Apoptosis, Oxidative phosphorylation, NDUFV1

Chen Li
contact affiliation	Shanghai Jiaotong University School of Medicine
contact email	cli@shsmu.edu.cn
lab head
Chen Li
contact affiliation	Shanghai Jiaotong University School of Medicine
contact email	cli@shsmu.edu.cn
dataset submitter

iProX dataset URI