PXD075100-1

PXD075100 is an original dataset announced via ProteomeXchange.

Title	Polyunsaturated aldehydes induce distinct proteomic responses in two diatom-associated bacterial communities
Description	Diatom-derived polyunsaturated aldehydes (PUAs) significantly influence marine bacterial dy-namics, yet the underlying proteomic mechanisms remain elusive. We employed high-resolution comparative proteomics to decipher the functional reprogramming of two bacterial communi-ties—one naturally associated with a PUA-producing diatom (N-community) and another with a non-PUA producer (I-community)—under ecologically relevant PUA exposure. While growth rates and cell densities remained unaffected, indicating an absence of acute toxicity, proteomics revealed pronounced community-specific reorganization. N-communities displayed stable, regula-tion-oriented adjustments consistent with physiological accommodation, whereas I-communities exhibited dose-dependent stress responses, shifting toward protein repair and antioxidant defense. Our findings demonstrate that PUAs trigger profound proteomic reprogramming conditioned by the communities' prior ecological history. This functional divergence provides a molecular basis for understanding bacterial fitness and succession during diatom blooms, where PUA-mediated in-teractions could act as a selective filter shaping the phycosphere's microbial landscape. Polyunsaturated aldehydes (PUA) produced by diatoms have been proposed to exert a wide range of effects on marine bacteria, from inhibitory or stress-inducing responses to neutral or potentially beneficial effects. However, the bacterial proteomic responses remain elusive. Here, we employed a high-resolution comparative proteomic approach to decipher the functional reprogramming of two distinct bacterial communities under ecologically relevant PUA exposure. One community was composed by bacteria naturally associated with a PUA-producing diatom (N- communy and, a second community associated with a non-PUA-producing diatom (I-community). Bacterial growth rates and final cell densities were not significantly affected by any treatment, indicating the absence of toxic effects even at high PUA concentrations. Dissolved organic carbon consumption did not provide evidence that PUA was the relevant carbon source. Interestingly, comparative proteomic analyses revealed pronounced community-specific reorganization in response to PUA expo-sure.Our results show that PUAs trigger a profound proteomic reprogramming rather than a simple stress response. While I-community prioritized antioxidant defense and protein repair, N-community showed a metabolic shift towards energy conservation. These findings suggest that the metabolic history of bacterial assemblages determines their success in the phycosphere, providing a molecular basis for microbial succession during diatom blooms.
HostingRepository	PRIDE
AnnounceDate	2026-06-04
AnnouncementXML	Submission_2026-06-04_12:54:10.573.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD075100
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ALMUDENA ESCOBAR NIÑO
SpeciesList	scientific name: Bacteria; NCBI TaxID: NEWT:2;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-02 11:47:48	ID requested
⏵ 1	2026-06-04 12:54:11	announced

10.6019/PXD075100;

10.3389/FMICB.2026.1838042;

submitter keyword: proteomics,diatom-bacteria interactions

microbial ecology

polyunsaturated aldehydes

phycosphere

Francisco Javier Fernández Acero
contact affiliation	University of Cádiz/IVAGRO
contact email	franciscojavier.fernandez@gm.uca.es
lab head
ALMUDENA ESCOBAR NIÑO
contact affiliation	University of Cadiz
contact email	almudena.escobar@uca.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075100
     1. Label: PRIDE project
     2. Name: Polyunsaturated aldehydes induce distinct proteomic responses in two diatom-associated bacterial communities