PXD075076-1

PXD075076 is an original dataset announced via ProteomeXchange.

Title	Loss of Drosophila nucleostemin 1 disrupts ribosomal protein homeostasis and rRNA processing to trigger apoptosis via the Xrp1/Irbp18 complex
Description	In this study, we investigate the role of Ns1 in proliferating epithelial tissues using the Drosophila wing imaginal disc as a model. We demonstrate that knocking down Ns1 in wing discs leads to strong apoptosis. However, unlike the reported effect on overexpressed RPs, we did not observe nucleolar accumulation of endogenous RPs upon Ns1 knockdown. Instead, proteomic analysis and the following validation with Western blotting and immunostaining reveal that Ns1 deficiency reduces the protein levels of most RPs without affecting their transcription or proteasomal degradation. We further demonstrate that loss of Ns1 upregulates the Xrp1/Irbp18 complex, which subsequently induces apoptosis through activating JNK and suppresses translation through a JNK-independent mechanism
HostingRepository	iProX
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_23:39:36.042.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiuxiu Liu
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-01 23:09:02	ID requested
⏵ 1	2026-03-01 23:39:36	announced

Dataset with its publication pending

submitter keyword: nucleostemin 1, ribosome biogenesis, Xrp1, Irbp18, JNK

Gongping Sun
contact affiliation	Shandong University
contact email	sgp@sdu.edu.cn
lab head
Xiuxiu Liu
contact affiliation	Shandong University
contact email	xiuxiuliu214@126.com
dataset submitter

iProX dataset URI