PXD075073 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Teneurin-4 switches between self-recognition and canonical Latrophilin binding to direct neuronal migration |
| Description | Cortical migration is a complex process in which neurons migrate along radial glial cells (RGC) to form functional layers. Teneurins (Ten1-4) play a role by interacting with Latrophilins (Lphn/ADGRL1-3). Teneurins are also known as cell adhesion molecules, but how homophilic and heterophilic Teneurin interactions are integrated is unknown. Here, single-particle-cryo-EM data of Ten2 shows that canonical Latrophilin-binding is sterically incompatible with Ten2-dimerisation, making these interactions exclusive. We engineered surface mutations that specifically disrupt Ten2-Ten2 or Ten2-Latrophilin interactions. These are transferrable to Ten4, suggesting conserved binding mechanisms. Proteomics, in-vivo-gene-editing and super-resolution-microscopy show that Ten4 is expressed along RGC fibres and that migrating neurons switch from low-to-high Ten4-expression. Ten4 expression is highest in the cortical plate where Ten4-Ten4 interactions reduce RGC-attachment. In the intermediate zone, Ten4-Latrophilin interactions are required to promote neuron-RGC association. The results show how Ten4 orchestrates cortical migration by exclusive structural mechanisms, underpinning the integration of distinct migration programmes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-28_16:26:05.858.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Barbara Steigenberger |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-01 20:12:03 | ID requested | |
| ⏵ 1 | 2026-06-28 16:26:06 | announced | |
Publication List
| 10.1038/s41467-026-71619-1; |
| Berbeira-Santana M, Peregrina C, Okuda K, Zhou JC, Carrasquero-Ordaz M, Roberts AV, Thomas AE, Haanappel E, Chavent M, El Omari K, Baker LA, Pederick DT, Pardon E, Steyaert J, N, รค, gerl UV, Del Toro D, Seiradake E, Structurally exclusive Teneurin complexes orchestrate divergent programs in early cortical development. Nat Commun, 17(1):(2026) [pubmed] |
Keyword List
| submitter keyword: cortex development,Teneurin, adhesion, neuronal migration, repulsion |
Contact List
| Daniel del Toro1 |
|---|
| contact affiliation | Dept Biomedical Sciences, School of Medicine and Health Sciences, Institute of Neurosciences, Universitat de Barcelona, E-08036 Barcelona |
| contact email | danieldeltoro@ub.edu |
| lab head | |
| Barbara Steigenberger |
|---|
| contact affiliation | MPI of Biochemistry |
| contact email | steigenberger@biochem.mpg.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075073
- Label: PRIDE project
- Name: Teneurin-4 switches between self-recognition and canonical Latrophilin binding to direct neuronal migration
