PXD075040 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Genetic and pharmacological inactivation of peptidoglycan remodeling increases antibiotic susceptibility of vancomycin-resistant Enterococcus faecium |
| Description | Vancomycin-resistant Enterococcus faecium (VREfm) is a leading cause of healthcare-associated infections globally and demands new approaches for treatment. Here we show that genetic and pharmacological inactivation of a highly conserved NlpC/P60 peptidoglycan hydrolase, secreted antigen A (SagA), enhanced vancomycin susceptibility of VREfm ex vivo and in vivo. Notably, genetic deletion of sagA impaired VREfm peptidoglycan remodeling, growth and increased the activity of vancomycin. We then identified first-in-class covalent NlpC/P60 peptidoglycan hydrolase inhibitors and demonstrated that pharmacological inactivation of SagA activity also impaired peptidoglycan remodeling and increased the efficacy of vancomycin in several VREfm clinical isolates. Our study reveals peptidoglycan hydrolases are druggable targets whose inactivation improves the efficacy of vancomycin against VREfm. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-13 |
| AnnouncementXML | Submission_2026-04-13_14:45:30.030.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kyong Fam |
| SpeciesList | scientific name: Enterococcus faecium ERV165; NCBI TaxID: NEWT:1134822; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-28 00:29:23 | ID requested | |
| ⏵ 1 | 2026-04-13 14:45:31 | announced | |
Publication List
| 10.1101/2025.09.11.675460; |
| Fam KT, Chodisetti PK, Wang Z, Homer JA, Smedley CJ, Kitamura S, Silva B, Xiong Y, Hansel-Harris A, Holcomb M, Babarinde S, Turner AM, Van Tyne D, Wilson IA, Forli S, Cravatt BF, Park D, Wolan DW, Moses JE, Hang HC, . bioRxiv, ():(2026) [pubmed] |
Keyword List
| submitter keyword: antibiotic adjuvant, covalent inhibitor, chemoproteomics,vancomycin-resistant Enterococcus faecium, peptidoglycan hydrolase |
Contact List
| Howard C. Hang |
|---|
| contact affiliation | Department of Immunology & Microbiology, Scripps Research, USA |
| contact email | hhang@scripps.edu |
| lab head | |
| Kyong Fam |
|---|
| contact affiliation | Scripps Research |
| contact email | tfam@scripps.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075040 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075040
- Label: PRIDE project
- Name: Genetic and pharmacological inactivation of peptidoglycan remodeling increases antibiotic susceptibility of vancomycin-resistant Enterococcus faecium
