⮝ Full datasets listing
PXD075031-1
PXD075031 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | IP-MS raw data of proteins precipitated by GLUD1/Igg in A549/DTX |
| Description | Nuclear protein extracts from tumor cells were prepared using a commercial extraction kit (Beyotime #P0027), quantified by BCA assay, and adjusted to 5 μg/μL in PBS containing 100 μM PMSF. For immunoprecipitation, 5 μg of anti-GLUD1 or control IgG antibody was incubated with nuclear lysates overnight at 4°C, followed by 4 h incubation with magnetic beads. Beads were washed (3× PBS) and proteins eluted in loading buffer (95°C, 5 min). Samples were resolved on 10% Omni-PAGE™ Hepes Plus gels, with excised bands processed for proteomics Analysis (APT Biotechnology). In brief, gel slices were reduced/alkylated, trypsin-digested (1:50 enzyme:protein, 37°C, 20 h), desalted, and analyzed by nano LC-MS/MS using a Q-Exactive HF-X system with a 120-min gradient (5-35% acetonitrile in 0.1% formic acid). MS parameters included: 70,000 resolution (MS1), 17,500 resolution (MS2), top-20 data-dependent acquisition, 27 eV HCD energy, and 30 s dynamic exclusion. Data were processed using MaxQuant 1.6.14 against the UniProt human database with standard search parameters (1% FDR). |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-28 |
| AnnouncementXML | Submission_2026-02-27_23:10:08.997.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiawen Cui |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-27 23:09:55 | ID requested | |
| ⏵ 1 | 2026-02-27 23:10:09 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: IP-MS |
Contact List
| Jiali Liu | |
|---|---|
| contact affiliation | China Pharmaceutical University |
| contact email | 897813761@qq.com |
| lab head | |
| Jiawen Cui | |
| contact affiliation | China Phramaceutical University |
| contact email | cuijw0514@outlook.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
