PXD075019-1

PXD075019 is an original dataset announced via ProteomeXchange.

Title	N-terminomic profiling identifies N-terminal acetylation abnormalities in NAA10p S37P cardiac organoids
Description	NAA10 serves as the catalytic subunit for both the NatA and N-terminal acetyltransferase E (NatE) complexes. To investigate the impact of the NAA10p.S37P mutation on N-terminal acetylation in COs, we employed the HUNTER strategy[小刘7.1][MS7.2] to enrich acetylated N-terminal peptides. Briefly, free amines of internal (non-N-terminal) tryptic peptides were modified with undecanal after trypsin digestion. This increased non-N-terminal peptide hydrophobicity, allowing them to be retained on a reverse-phase chromatography column, while N-terminal acetylation peptides were not retained. These peptides were further purified by C18 solid-phase extraction. After drying in a SpeedVac concentrator, the samples were reconstituted in 0.1% TFA and subjected to LC–MS/MS analysis.
HostingRepository	iProX
AnnounceDate	2026-02-28
AnnouncementXML	Submission_2026-02-27_19:11:48.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mingwei Sun
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-27 19:11:21	ID requested
⏵ 1	2026-02-27 19:11:48	announced

Dataset with its publication pending

submitter keyword: NAA10p.S37P, N-terminomic profiling, N-terminal acetylation

Huiming Yuan
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	Yuan_huiming@gibh.ac.cn
lab head
Mingwei Sun
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	sun_mingwei@gibh.ac.cn
dataset submitter

iProX dataset URI