PXD074975-1

PXD074975 is an original dataset announced via ProteomeXchange.

Title	Proteomic Characterization of SMARCA-Deficient Esophageal Adenocarcinoma Reveals Complement Evasion and DNA Repair-Associated Prognostic Subgroups
Description	Background Esophageal adenocarcinoma (EAC) is an aggressive malignancy in which the SWI/SNF catalytic subunits SMARCA2 and SMARCA4 are frequently lost, yet the biological and clinical impact of these deficiencies remains unclear. Methods We analyzed 113 EAC specimens using mass spectrometry–based proteomics, including 89 SMARCA-deficient and 24 SMARCA-proficient tumors. Additional immunohistochemical studies were conducted on 98 cases. Differential expression, pathway enrichment, and survival analyses were used to characterize proteomic alterations and identify prognostic features. Results SMARCA-deficient tumors showed frequent expression of complement-inactivating proteins CD55 and CD59 with distinctive apical and membranous localization. SMARCA4-deficient patients had significantly poorer survival than both SMARCA2-deficient and SMARCA-proficient patients. Among neoadjuvantly treated SMARCA-deficient tumors, coordinated upregulation of DNA repair proteins separated patients into two prognostic subgroups. The DNA repair–low subgroup had markedly worse survival than both the DNA repair–high subgroup and SMARCA-proficient controls, whereas the DNA repair–high subgroup showed a trend toward improved outcomes. This DNA repair–associated pattern was not observed in neoadjuvantly treated SMARCA-proficient tumors. Conclusions SMARCA-deficient EAC comprises biologically distinct subgroups with prognostic proteomic signatures. CD55 and CD59 expression may contribute to resistance to antibody-based immunotherapies by complement evasion, while DNA repair profiles could guide post-surgical treatment in neoadjuvantly treated patients.
HostingRepository	PRIDE
AnnounceDate	2026-06-25
AnnouncementXML	Submission_2026-06-25_01:31:34.807.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-27 00:24:25	ID requested
⏵ 1	2026-06-25 01:31:35	announced

Dataset with its publication pending

submitter keyword: Esophageal adenocarcinoma

SWI/SNF complex

Proteomics

SMARCA2

SMARCA4

Complement system

CD55

CD59

DNA repair

Alexander Quaas
contact affiliation	Institut für Pathologie Uniklinik Köln, Kerpener Straße 62, 50937 Cologne, Germany
contact email	alexander.quaas@uk-koeln.de
lab head
Proteomics Facility
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074975
     1. Label: PRIDE project
     2. Name: Proteomic Characterization of SMARCA-Deficient Esophageal Adenocarcinoma Reveals Complement Evasion and DNA Repair-Associated Prognostic Subgroups