PXD074900 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Sup:EasySCP Unveils Extensive Liver Zonation at Single-cell Proteomics Resolution |
| Description | The widespread adoption of single-cell proteomics (SCP) in biology has been limited by complex workflows and reliance on specialized instrumentation. Here we present EasySCP, a high-throughput and lossless method that integrates FACS-based single-cell sorting, an all-in-one, single-step digestion process in 384-well plates, and sensitive mass spectrometry. EasySCP identifies nearly 5,000 proteins from individual HEK293 cell. Applied to murine liver, EasySCP achieved spatially resolved proteomics profiling of hepatocytes zonation, detecting an average of 3,500 proteins per hepatocyte and uncovering zonation patterns for 3,277 out of 5,267 proteins, representing unprecedented depth and coverage in single-cell liver proteomics. Building on 215 conserved zonation markers, we further developed hepatocyte spatial status score (HSS) that enables accurately reconstruct liver zonation across single-cell and spatial multi-omics datasets. Together, our study introduces EasySCP, a broadly accessible tool for dissecting cellular heterogeneity at single-cell proteomics resolution in both healthy and disease states, effectively bridging the gap between transcriptomics and functional proteomics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-06 |
| AnnouncementXML | Submission_2026-05-05_23:10:52.506.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bingbing hao |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral Zoom |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-25 10:23:21 | ID requested | |
| ⏵ 1 | 2026-05-05 23:10:53 | announced | |
Publication List
Keyword List
| submitter keyword: Hela cell |
Contact List
| Bingbing Hao |
|---|
| contact affiliation | Zhengzhou University |
| contact email | bbhao@zzu.edu.cn |
| lab head | |
| Bingbing hao |
|---|
| contact affiliation | Zhengzhou University |
| contact email | bbhao@zzu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074900 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074900
- Label: PRIDE project
- Name: Sup:EasySCP Unveils Extensive Liver Zonation at Single-cell Proteomics Resolution
