PXD074890 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | UFMylation-Dependent Inhibition of AKT Signaling by PHLDA3 in Lung Adenocarcinoma |
| Description | UFMylation, a recently identified ubiquitin-like modification mediated by the E3 ligase UFL1, plays context-specific roles in cancers, but its substrates and functions in lung adenocarcinoma (LUAD) remain poorly defined. Here, we identify the AKT signaling repressor PHLDA3 as a substrate of UFL1 in LUAD. UFMylation of PHLDA3 at Lys51 and Lys106 promotes its membrane localization, thereby blocking AKT membrane recruitment and suppressing downstream signaling. Tumor-associated PHLDA3 mutations F41L, E82G and K106N impair its UFMylation and membrane translocation, resulting in AKT hyperactivation and enhanced tumor growth. In samples from patients with LUAD, UFL1 expression inversely correlates with phosphor-AKT levels. Functionally, the UFL1-PHLDA3 axis inhibits LUAD progression in both cell line-based and patient-derived xenograft models. These findings define a tumor-suppressive UFMylation pathway that modulates AKT activity and provides a mechanistic rationale for targeting UFL1-PHLDA3 signaling in LUAD. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-08 |
| AnnouncementXML | Submission_2026-04-08_04:25:30.279.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiuqing Ma |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-25 03:21:35 | ID requested | |
| ⏵ 1 | 2026-04-08 04:25:30 | announced | |
Publication List
Keyword List
Contact List
| Tongzheng Liu |
|---|
| contact affiliation | State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China/College of Pharmacy, Jinan University, Guangzhou, 510632, China |
| contact email | liutongzheng@jnu.edu.cn |
| lab head | |
| Xiuqing Ma |
|---|
| contact affiliation | Jinan University |
| contact email | maxiuqing@stu2024.jnu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074890 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074890
- Label: PRIDE project
- Name: UFMylation-Dependent Inhibition of AKT Signaling by PHLDA3 in Lung Adenocarcinoma
