PXD074784-1

PXD074784 is an original dataset announced via ProteomeXchange.

Title	An Amphibious Bi-functional Probe For Protein Chemical Cross-linking.
Description	Chemical cross-linking mass spectrometry (CXMS) has emerged as a powerful and well-established method for probing protein structure, conformational dynamics, and protein–protein interactions, particularly in cases where classical high-resolution techniques face intrinsic limitations. The development of new cross-linking reagents with defined reactivity, acti-vation, and compatibility with native conditions remains essential for expanding the scope and reliability of CXMS anal-yses. Here we report the design and synthesis of two novel bis-hypervalent iodine reagents, called Togni cross-linking rea-gents 1 and 2, which function as efficient covalent protein cross-linkers. These reagents feature hypervalent iodide motifs on both termini, enabling intra- and intermolecular cross-link formation. They selectively target aromatic amino acids and cys-teine residues and can be activated either by ascorbic acid or through hydrogen abstraction from thiol groups of cysteines. The performance of both reagents and activation modes is demonstrated on a set of structurally and functionally diverse proteins, including apomyoglobin/holomyoglobin and the small GTPase RHOA. Together, these results establish Togni cross-linking agents as versatile additions to the CXMS toolbox and highlight the potential of bis-hypervalent iodine chemistry for studying protein structure and dynamics under mild aqueous conditions.
HostingRepository	PRIDE
AnnounceDate	2026-07-13
AnnouncementXML	Submission_2026-07-12_16:09:49.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Karpisek
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-24 13:53:47	ID requested
⏵ 1	2026-07-12 16:09:50	announced

10.1021/jacs.6c04886;

Karp, í, š, ek M, Fojt, í, k L, Fiala J, Langer V, Matou, š, ek V, Kuka, č, ka Z, Nov, á, k P, An Amphibious Bifunctional Probe for Protein Chemical Cross-Linking. J Am Chem Soc, 148(28):29883-29894(2026) [pubmed]

submitter keyword: Togni reagents, radical labeling, chemical cross-linking, mass spectrometry, protein footprinting, fluoroalkylation,: protein structure

Petr Novak
contact affiliation	Institute of Microbiology, The Czech Academy of Sciences
contact email	pnovak@biomed.cas.cz
lab head
Michael Karpisek
contact affiliation	Institute of Microbiology of the Czech Academy of Sciences, 14220 Prague, Czech Republic
contact email	michael.karpisek@biomed.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD074784

PRIDE project URI

• PRIDE
  1. PXD074784
     1. Label: PRIDE project
     2. Name: An Amphibious Bi-functional Probe For Protein Chemical Cross-linking.