PXD074712 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Break-induced replication is enhanced by a phospho-activated RPA-binding module in Pol32 |
| Description | Break-induced replication (BIR) mediates single-ended DNA double-strand break (DSB) repair. Upon strand invasion into a homologous repair template, BIR is catalysed by a minimal replisome comprising PCNA, DNA polymerase delta, and the Pif1 helicase. Here, we identify an interaction between Polymerase delta and single-stranded DNA (ssDNA)-binding protein RPA mediated by an RPA-binding module (RBM) within the Polymerase delta subunit Pol32 and RPA subunit Rfa1. Pol32 RBM phosphorylation at Thr256 and Thr257 increases its affinity for Rfa1, while corresponding phospho-mimetic amino-acid substitutions promote BIR efficiency in vivo. This suggests that Pol32 functions as a rheostat whose phosphorylation enhances the affinity of Polymerase delta for RPA-bound BIR intermediates, thereby boosting BIR efficiency. Modelling indicates that Pol32 phospho-RBM-Rfa1 interactions mirror the binding mode of a previously identified Pif1 RBM, and an RBM we identify in the BIR antagonist Mph1. This implies a key role for RPA in the dynamic orchestration of the enzymes mediating BIR. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-15 |
| AnnouncementXML | Submission_2026-06-14_16:11:21.724.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rowin Appanah |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-21 07:37:00 | ID requested | |
| ⏵ 1 | 2026-06-14 16:11:22 | announced | |
Publication List
| Jones D, Appanah R, Yates LA, Oliver AW, Rass U, Break-induced replication is enhanced by a phospho-activated RPA-binding module in Pol32. Nat Commun, 17(1):(2026) [pubmed] |
| 10.1038/s41467-026-71309-y; |
Keyword List
| submitter keyword: Cdc5, break-induced replication, Vanquish Neo UHPLC, DDK, Orbitrap Exploris 480, Pol32, Cka2,Saccharomyces cerevisiae |
Contact List
| Ulrich Rass |
|---|
| contact affiliation | Professor of Genome Stability, Genome Damage and Stability, School of Life Sciences, University of Sussex. Brighton. BN1 9RH. |
| contact email | U.W.Rass@sussex.ac.uk |
| lab head | |
| Rowin Appanah |
|---|
| contact affiliation | 1.University of Sussex
2.LBMC-ENS de Lyon |
| contact email | rappanah@live.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074712
- Label: PRIDE project
- Name: Break-induced replication is enhanced by a phospho-activated RPA-binding module in Pol32
