PXD074707-1

PXD074707 is an original dataset announced via ProteomeXchange.

Title	Efficient plasmid-based rescue of T7 RNA polymerase-driven calicivirus reverse genetics systems in mammalian cells using vaccinia virus RNA capping enzymes C4PR_LIV
Description	Calicivirus reverse genetics systems have typically relied on either in vitro transcribed RNA or plasmid-based rescue with enzyme supplementation. Plasmid based calicivirus rescue relies on supplementation with Fowl Pox T7 RNA polymerase which has cost and labour considerations. Here we present a novel system integrating vaccinia capping enzymes D1R and D12L encoded on plasmids in a rescue system for Murine Norovirus (MNV). Addition of D1R, D12L and T7 expressing plasmids increases viral rescue tires of MNV in both BSR-T7 cells and transgenic BSR-T7CD300LF cells and viral polyprotein abundance. This system offers an opportunity to increase viral rescue efficiency for MNV, other caliciviruses and permit higher throughput experimentation such as mutagenic screening.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_08:49:58.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leandro Neves
SpeciesList	scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: NEWT:10036; scientific name: Murine norovirus; NCBI TaxID: NEWT:357231;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-21 00:37:04	ID requested
⏵ 1	2026-05-13 08:49:59	announced

10.3390/V18050536;

submitter keyword: RNA capping,Calicivirus, Norovirus, Reverse Genetics

Dr Ed Emmott
contact affiliation	Centre for Proteome Research, Dept. Biochemistry & Systems Biology, University of Liverpool, Liverpool, UK
contact email	E.Emmott@liverpool.ac.uk
lab head
Leandro Neves
contact affiliation	Centre for Proteome Research, Department of Biochemistry, Cell & Systems Biology, Institute of Systems, Molecular & Integrative Biology, University of Liverpool, Liverpool, UK
contact email	leandroxneves@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074707
     1. Label: PRIDE project
     2. Name: Efficient plasmid-based rescue of T7 RNA polymerase-driven calicivirus reverse genetics systems in mammalian cells using vaccinia virus RNA capping enzymes C4PR_LIV