PXD074689-1

PXD074689 is an original dataset announced via ProteomeXchange.

Title	Cryptosporidium secreted proteins form a complex layered interface with the host cell
Description	Cryptosporidium parvum, one of the leading causes of diarrheal death in children, remodels its infection site through secreted effector proteins. Several of them accumulate at the host-parasite interface, forming a complex structure whose function and importance for infection remain poorly understood. Here, we localized and functionally characterized the putative dense granule protein DG8. We confirmed the protein to be in the dense granules, forming a ring structure once secreted at the host-parasite interface. Deletion of DG8 showed reduced infection in mice in vivo and early defect in vitro, revealing the importance of DG8 for parasite fitness. Five additional secreted proteins were identified as potential partners, among which SG4, a small granule protein, was shown to partially co-localize with DG8. Applying ultrastructure expansion microscopy on intestinal sections with newly generated antibodies against DG8 and SG4, we could precisely position the ring structure above the dense band, around the feeder organelle at the base of the parasitophorous vacuole membrane with an unprecedent resolution. Altogether, better visualization and understanding of the host-parasite interface through expanded intestinal tissue and effectors characterization reveal a complex and essential layered host-parasite interface.
HostingRepository	PRIDE
AnnounceDate	2026-05-04
AnnouncementXML	Submission_2026-05-04_05:50:30.414.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD074689
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Amandine Guerin
SpeciesList	scientific name: Cryptosporidium parvum Iowa II; NCBI TaxID: NEWT:353152;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-20 08:43:24	ID requested
⏵ 1	2026-05-04 05:50:31	announced

10.6019/PXD074689;

submitter keyword: small granules,cryptosporidium, dense granules, DG8

Amandine Guérin
contact affiliation	Department of Microbiology and Molecular Medicine, Faculty of Medicine,University of Geneva
contact email	amandine.guerin@unige.ch
lab head
Amandine Guerin
contact affiliation	University of Geneva
contact email	amandine.guerin@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074689
     1. Label: PRIDE project
     2. Name: Cryptosporidium secreted proteins form a complex layered interface with the host cell