PXD074672-1

PXD074672 is an original dataset announced via ProteomeXchange.

Title	Site-Specific Glycosylation Profiling of Protein Subunit and Inactivated Virus Vaccines
Description	Many protein subunit vaccines and biologics contain glycosylated antigens and antibodies, yet quantitative frameworks for comparing glycosylation across lots, manufacturers, and production platforms remain limited. We performed site specific glycosylation LC-MS/MS analysis for intact N-linked glycopeptides on licensed protein subunit vaccines, including influenza from egg-, MDCK-, and Sf9-derived products, plus Sf9 SARS-CoV-2 spike and CHO varicella-zoster virus glycoprotein E. Glycopeptide abundance distribution spectra were compared using the NIST MS Search dot-product score to quantify similarity of site-specific glycan distributions. Across all sites, distributions clustered into six recurrent classes (two high-mannose, three core-fucosylated complex, and one multi-sialylated complex profile). Replicate analyses, year-to-year formulations, and different manufacturers within the same platform were highly similar (scores 978, 961, and 960 out of 999), whereas intra-protein sites, influenza inter-strain sites, and different production sources were less similar (scores 554, 540, and 209). Interestingly, glycosylation differences between production sources tracked phylogenetic relatedness of host species.
HostingRepository	PRIDE
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-21_08:47:06.896.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zachary Goecker
SpeciesList	scientific name: Influenza A virus (A/Panama/2007/1999(H3N2)); NCBI TaxID: NEWT:381513; scientific name: Influenza A virus (A/Shandong/9/1993(H3N2)); NCBI TaxID: NEWT:1144025; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: NEWT:2697049; scientific name: Human alphaherpesvirus 3; NCBI TaxID: NEWT:10335; scientific name: Influenza A virus (A/Philippines/2/1982(H3N2)); NCBI TaxID: NEWT:382825; scientific name: Influenza A virus (A/New Caledonia/20/1999(H1N1)); NCBI TaxID: NEWT:381512;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-20 00:06:56	ID requested
⏵ 1	2026-07-21 08:47:07	announced

Dataset with its publication pending

submitter keyword: Glycosylation, Mass spectrometry, Site-specific,Vaccines, Proteomics

Stephen E. Stein
contact affiliation	National Institute of Standards and Technology
contact email	stephen.stein@nist.gov
lab head
Zachary Goecker
contact affiliation	National Institute of Standards and Technology
contact email	zachary.goecker@nist.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074672
     1. Label: PRIDE project
     2. Name: Site-Specific Glycosylation Profiling of Protein Subunit and Inactivated Virus Vaccines