PXD074667-1

PXD074667 is an original dataset announced via ProteomeXchange.

Title	Identification of HSP70 family proteins as targets of anti-melanosome monoclonal antibodies by immunoprecipitation and LC–MS/MS
Description	This dataset contains proteomic analyses performed to identify the molecular targets of three anti-melanosome monoclonal antibodies (mAbs): Mel321-31, Mel322-34, and Mel321-35. These antibodies were generated following immunization of mice with purified melanosomes and were shown to bind melanosomes by ELISA and flow cytometry. To determine their protein targets, melanosomal lysates were subjected to immunoprecipitation using each mAb. An isotype control antibody (mGO.53), which does not bind melanosomes, was used to define background binding. Each antibody condition was analyzed in four independent replicates (n=4), resulting in a total of 16 samples (3 experimental antibodies + 1 isotype control). Enriched proteins were identified by label-free quantitative LC–MS/MS and statistically compared to the isotype control group. The analysis revealed significant enrichment of HSP70 family members (HspA1A, HspA1L, HspA5, and HspA8), identifying HSP70 proteins as the primary targets of the anti-melanosome mAbs.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:14:45.309.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noam Ben Shalom
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-19 21:00:02	ID requested
⏵ 1	2026-02-22 17:14:45	announced

Dataset with its publication pending

submitter keyword: None

Natalia T
contact affiliation	Department of Microbiology and Immunology Faculty of Medicine Tel Aviv University Tel Aviv, Israel
contact email	nfreund@tauex.tau.ac.il
lab head
Noam Ben Shalom
contact affiliation	Gray School of Medical Sciences, Gray Faculty of Medical & Health Sciences, Tel Aviv University, 6997801 Israel.
contact email	noam370@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD074667

PRIDE project URI

• PRIDE
  1. PXD074667
     1. Label: PRIDE project
     2. Name: Identification of HSP70 family proteins as targets of anti-melanosome monoclonal antibodies by immunoprecipitation and LC–MS/MS