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PXD074647-1
PXD074647 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | KRAS4A Promotes Oligomerization of Hexokinase 1 on Mitochondria |
| Description | Among the ways by which oncogenic KRAS upregulates glycolysis in cancer is direct interaction of KRAS4A with Hexokinase 1 (HK1), but the mechanism is unknown. HK1 associates with the outer mitochondrial membrane (OMM) where its allosteric regulation depends on homodimerization. Using affinity capture, FRET and blue native gels we show that KRAS4A enhances oligomerization of HK1 on the OMM. Modeling the HK1/KRAS4A complex with AlphaFold 3 predicts that the membrane association sequences of both HK1 and KRAS4A are oriented toward the OMM. Super-resolution microscopy showed colocalization of HK1 and KRAS4A on the OMM with HK1 enriched at discrete locations. Single-molecule tracking reveals HK1 diffusing freely along the OMM and dwelling at discrete regions where two molecules can be seen to colocalize transiently. KRAS4A expression decreased the diffusion coefficient of HK1 on the organelle. Thus, KRAS4A alters the dynamics of HK1 on the OMM and enhances its activity by promoting oligomerization. The raw mass spectrometric files that confirm the association of KRAS4A with HK1 complexes on the outer mitochondrial membrane are presented here. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-19 |
| AnnouncementXML | Submission_2026-02-19_14:29:05.211.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Beatrix Ueberheide |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Deamidated |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-19 14:16:03 | ID requested | |
| ⏵ 1 | 2026-02-19 14:29:05 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Oligomerization of Hexokinase 1 on OMM, KRAS4A Hexokinase interaction, DatasetType:Proteomics |
Contact List
| Beatrix M Ueberheide | |
|---|---|
| contact affiliation | NYU Langone Grossman School of Medicine |
| contact email | beatrix.ueberheide@nyulangone.org |
| lab head | |
| Beatrix Ueberheide | |
| contact affiliation | NYU School of Medicine |
| contact email | beatrixueberheide@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100905/ |
