PXD074581-1

PXD074581 is an original dataset announced via ProteomeXchange.

Title	A pore-forming toxin initiates ABI1 complex switching to promote bacterial cell-to-cell spread
Description	Listeria monocytogenes (Lm), a prototypical intracellular bacterial pathogen, expresses ActA to initiate ARP2/3-mediated actin-based motility in the cytosol of host cells. Motile bacteria generate Lm-containing protrusions at the cell surface, facilitating direct cell-to-cell spread and dissemination within the host. Protrusion formation is an active, spatially regulated process, yet how Lm coordinates bacterial and host factors to orchestrate this process remains unclear. Here, we identify Abelson-interactor 1 (ABI1) as a host factor required for efficient Lm protrusion formation and cell-to-cell spread. Conditional knockout of Abi1 in mice significantly reduces susceptibility to Lm infection, while deletion of actA abrogates the protective effect of Abi1 knockout. During Lm infection, ABI1 is uncoupled from spectrin at the cell cortex and binds to EPS8 within protrusions. This ABI1 “complex switching” is initiated by the pore-forming toxin LLO, which perforates the host plasma membrane and triggers Ca2+ influx, leading to calpain-mediated cleavage of the spectrin cytoskeleton. Spectrin cleavage mobilizes ABI1, allowing ABI1 to bind EPS8 and activate EPS8’s actin capping activity to facilitate local actin recycling necessary for efficient protrusion elongation and cell-to-cell spread. These findings reveal an unrecognized host-pathogen interaction, in which a bacterial pore-forming toxin induces spatially confined cytoskeletal remodeling to promote cell-to-cell spread.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_16:11:16.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-18 00:06:40	ID requested
⏵ 1	2026-06-14 16:11:17	announced

10.1038/s41467-026-71510-z;

Sun H, Wizzard A, Brumell JH, Higgins DE, A pore-forming toxin initiates ABI1 complex switching to promote bacterial cell-to-cell spread. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: Cell-to-cell spread,Listeria, Infectious diseases, IP-MS

Darren Higgins
contact affiliation	Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts, USA
contact email	darren_higgins@hms.harvard.edu
lab head
He Sun
contact affiliation	Harvard Medical School
contact email	he_sun@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD074581

PRIDE project URI

• PRIDE
  1. PXD074581
     1. Label: PRIDE project
     2. Name: A pore-forming toxin initiates ABI1 complex switching to promote bacterial cell-to-cell spread