PXD074580-1

PXD074580 is an original dataset announced via ProteomeXchange.

Title	2-arachidonoylglycerol (2-AG) modulates the proteome and genes associated with the intestinal barrier of Caco-2 cells over the inflammatory response of IL-1β
Description	Traumatic brain injury (TBI) is a major risk factor for late-onset neurodegenerative disorders, including Alzheimer’s disease (AD). We investigated whether mild TBI (mTBI) at the presymptomatic stage differentially affects the gut microbiota–endocannabinoidome (eCBome) axis. Caco-2 cells were treated as follows: (i) untreated (CTRL), (ii) IL-1β (10 ng/ml for 24 h), (iii) 2-AG 10 μM (24 h), (iv) 2-AG 10 μM + IL-1β (30 min 2-AG IL-1β 24 h). We characterized proteome-wide quantitative changes in this cell model under different treatment conditions using the LFQ proteomics approach. Our findings identify the gut microbiota–endocannabinoidome axis as a key mediator of long-term outcomes after brain trauma.
HostingRepository	PRIDE
AnnounceDate	2026-07-31
AnnouncementXML	Submission_2026-07-31_02:15:25.273.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Costanzo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-18 00:03:23	ID requested
⏵ 1	2026-07-31 02:15:25	announced

Dataset with its publication pending

submitter keyword: traumtatic brain injury, Caco-2 cells,proteomics, gut microbiota-endocannabinoidome, 2-arachidonoylglycerol

Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II
contact email	michele.costanzo@unina.it
lab head
Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology - University of Naples Federico II
contact email	michele.costanzo@unina.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD074580

PRIDE project URI

• PRIDE
  1. PXD074580
     1. Label: PRIDE project
     2. Name: 2-arachidonoylglycerol (2-AG) modulates the proteome and genes associated with the intestinal barrier of Caco-2 cells over the inflammatory response of IL-1β