PXD074552-1

PXD074552 is an original dataset announced via ProteomeXchange.

Title	Amphiregulin drives mitochondrial vesicle packaging and delivery for immune modulation
Description	Amniotic Epithelial Cells (AECs) exhibit paracrine immunoregulatory functions, however the effectors within their extracellular vesicles (EVs) are poorly defined. Using label-free proteomics and genetic approaches, we profiled the secretome of ovine AECs under basal and lipopolysaccharide (LPS)-mimicking inflammatory stimuli. Microvesicles (MVs) emerged as the main LPS-responsive EVs, selectively enriching 169 proteins involved in immunometabolism and stress signaling. Among these, the Epidermal Growth Factor ligand Amphiregulin (AREG) was enriched in MVs after LPS. AREG silencing (siAREG) abolished mitochondria packaging into MVs impairing their delivery to immune cells. siAREG-MVs exhibited reduced uptake by Peripheral Blood Mononuclear Cells (PBMCs) and T-cells, diminishing their capacity to suppress proliferation and CD3-dependent NFAT activation. Supplementation with exogenous AREG partially restored mitochondrial transfer and immunosuppression, supporting a dual immunoregulatory role for AREG in AEC biology. These findings elucidate the immunomodulatory function of AEC-derived MVs and highlight AREG-mediated vesicular signaling as a promising immunotherapeutic target for cell-free strategies.
HostingRepository	PRIDE
AnnounceDate	2026-07-18
AnnouncementXML	Submission_2026-07-18_02:57:54.964.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adrián Cerveró Varona
SpeciesList	scientific name: Ovis aries; NCBI TaxID: NEWT:9940;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-17 08:44:42	ID requested
⏵ 1	2026-07-18 02:57:55	announced

Dataset with its publication pending

submitter keyword: Ovis Aries, amphiregulin, Evosep One, timsTOF Pro 2

Hélder A. Santos
contact affiliation	Faculty of Pharmacy, University of Helsinki University Medical Center Groningen / University of Groningen, The Netherlands
contact email	helder.santos@helsinki.fi
lab head
Adrián Cerveró Varona
contact affiliation	University of Teramo
contact email	acerverovarona@unite.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD074552

PRIDE project URI

• PRIDE
  1. PXD074552
     1. Label: PRIDE project
     2. Name: Amphiregulin drives mitochondrial vesicle packaging and delivery for immune modulation