PXD074548-1

PXD074548 is an original dataset announced via ProteomeXchange.

Title	Development of Proteome Derived Cyclic Peptide Libraries via Ortho Phthalaldehyde Assisted Cyclization
Description	This dataset contains raw LC–MS/MS data and associated search results generated for the identification and characterization of proteome-derived cyclic peptides produced via ortho-phthalaldehyde (OPA)-mediated macrocyclization. Cysteine-containing peptides derived from human proteome digests were selectively enriched and subjected to OPA-mediated intramolecular cyclization under mild aqueous conditions. The resulting cyclic peptide libraries were analyzed by high-resolution Orbitrap mass spectrometry. MS/MS data were processed using pLink3 with customized modification parameters corresponding to OPA-mediated cyclization products. The dataset includes raw Thermo .raw files and associated search results supporting the systematic annotation of cyclic peptide topologies and fragmentation behavior. These data support the development of a rule-based automated MS/MS annotation pipeline and the identification of functional cyclic peptide binders targeting human immunoglobulin G (IgG).
HostingRepository	PRIDE
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-02_18:55:28.206.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Wei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-17 07:28:39	ID requested
⏵ 1	2026-09-02 18:55:28	announced

Dataset with its publication pending

submitter keyword: Cyclic peptide library, mass spectrometry, ortho-phthalaldehyde (OPA), IgG-binding peptide, protein recognition

Haiteng Deng
contact affiliation	MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, State Key Laboratory of Complex, Severe, and Rare Diseases, School of Life Sciences, Tsinghua University
contact email	dht@tsinghua.edu.cn
lab head
Ying Wei
contact affiliation	Tsinghua University
contact email	wy19@tsinghua.org.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD074548

PRIDE project URI

• PRIDE
  1. PXD074548
     1. Label: PRIDE project
     2. Name: Development of Proteome Derived Cyclic Peptide Libraries via Ortho Phthalaldehyde Assisted Cyclization