PXD074521 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Analysis of the Protein Composition of Migrasomes Enriched by Magnetic Aptamer Probe System and Density Gradient Centrifugation |
| Description | This study aims to evaluate the performance of the magnetic aptamer probe (MAP) system in the enrichment of migrasomes. First, the MAP system was constructed by functionalizing biotin-labeled migrasome-targeting aptamers (Apt_B3) onto the surface of NeutrAvidin-coated magnetic sub-micrometre particles. To gain a deeper understanding of the system’s efficacy, we collected 4 clinical plasma samples and isolated the migrasomes using both traditional density gradient centrifugation (DGC) and the MAP system respectively. Subsequently, the protein composition of the migrasomes isolated by these two methods was analyzed using quantitative proteomics. To directly evaluate the reproducibility of our MAP isolation protocol, we compared the proteomic profiles of migrasomes isolated in two independent preparation batches. Batch-1 consisted of two replicates (MAP_a and MAP_b), and Batch-2 comprised four replicates (MAP_1-4). This approach seeks to demonstrate the promising applicability of the MAP system in studies related to clinical disease diagnosis of migrasomes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-04-26_16:10:59.502.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yixin Xu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-16 09:11:02 | ID requested | |
| ⏵ 1 | 2026-04-26 16:11:01 | announced | |
Publication List
| 10.1002/jev2.70283; |
| Xu Y, Jiao L, Yi Q, Chen Y, Cai B, Zhang J, Huang Z, Luo Y, Si Y, Wu Y, Ying B, Chen J, Zhou J, Magnetic-Assisted Aptamer Selection and Isolation of Migrasomes for Application in Lung Adenocarcinoma Proteomic Analysis. J Extracell Vesicles, 15(4):e70283(2026) [pubmed] |
Keyword List
| submitter keyword: Density Gradient Centrifugation, Proteomic, Aptamer,Migrasome |
Contact List
| Yixin Xu |
|---|
| contact affiliation | Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. |
| contact email | xuyixin@scu.edu.cn |
| lab head | |
| Yixin Xu |
|---|
| contact affiliation | Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. |
| contact email | xuyixin@scu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074521 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074521
- Label: PRIDE project
- Name: Proteomic Analysis of the Protein Composition of Migrasomes Enriched by Magnetic Aptamer Probe System and Density Gradient Centrifugation
