PXD074496-1

PXD074496 is an original dataset announced via ProteomeXchange.

Title	Cardiomyocyte Cyclin-dependent kinase 9 directly binds to and phosphorylates NF-κB p65 subunit to drive cardiac inflammation and remodeling
Description	Hypertensive heart failure highlights an urgent need for effective therapeutic strategies. Protein kinases regulate multiple pathways in cardiac pathophysiology and may provide promising therapeutic targets. Here, we identified a Cyclin-dependent kinase, CDK9, promoting inflammation and cardiac remodeling in terminally differentiated cardiomyocytes. Firstly, kinase enrichment analysis and experimental evidence revealed CDK9 phosphorylation at Thr-186 in both human and mouse hypertrophic heart tissues. CDK9 loss of function via T186A mutation in cardiomyocytes attenuated Ang II-induced heart remodeling and NF-κB-mediated inflammation, whereas CDK9 overactivation by T186E mutation induces. This regulatory function of CDK9 in cardiac remodeling is cell cycle-independent. Further studies demonstrate that the kinase domain of CDK9 directly binds to NF-κB P65 protein, which leads to the CDK9/P65 complex nuclear translocation, P65 phosphorylation, and transcription of inflammatory and hypertrophic genes in cardiomyocytes. This process requires CDK9 Thr-186 phosphorylation and Cyclin T1 presence, but is independent on IKKβ and CDK9-RNAPII pathways. Pharmacological inhibition of CDK9 phosphorylation significantly attenuated Ang II-induced cardiac inflammation, remodeling, and dysfunction in mice. Collectively, Ang II-activated CDK9 directly binds to and phosphorylates P65 to drive cardiac inflammation and remodeling. This study identifies CDK9 as a potential target in heart failure therapeutics
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_16:11:11.474.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	仕炬 叶
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-15 14:05:53	ID requested
⏵ 1	2026-06-14 16:11:12	announced

Ye S, Zhao Y, Tu H, Han X, Liu T, Gong Y, Lu J, Jin T, Luo W, Qu X, Lai D, Fu G, Liang G, B p65 subunit to drive cardiac inflammation and remodeling. Nat Commun, 17(1):(2026) [pubmed]

10.1038/s41467-026-70410-6;

submitter keyword: Angiotensin II

CDK9

NF-κB P65

Cardiac remodeling

Cardiomyocytes

Guang Liang
contact affiliation	Hangzhou Medical College
contact email	wzmcliangguang@163.com
lab head
仕炬 叶
contact affiliation	Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University
contact email	3324111@zju.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074496
     1. Label: PRIDE project
     2. Name: Cardiomyocyte Cyclin-dependent kinase 9 directly binds to and phosphorylates NF-κB p65 subunit to drive cardiac inflammation and remodeling