PXD074483 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cardiomyocyte Cyclin-dependent kinase 9 directly binds to and phosphorylates NF-κB p65 subunit to drive cardiac inflammation and remodeling |
| Description | Hypertensive heart failure highlights an urgent need for effective therapeutic strategies. Protein kinases regulate multiple pathways in cardiac pathophysiology and may provide promising therapeutic targets. Here, we identified a Cyclin-dependent kinase, CDK9, promoting inflammation and cardiac remodeling in terminally differentiated cardiomyocytes. Firstly, kinase enrichment analysis and experimental evidence revealed CDK9 phosphorylation at Thr-186 in both human and mouse hypertrophic heart tissues. CDK9 loss of function via T186A mutation in cardiomyocytes attenuated Ang II-induced heart remodeling and NF-κB-mediated inflammation, whereas CDK9 overactivation by T186E mutation induces. This regulatory function of CDK9 in cardiac remodeling is cell cycle-independent. Further studies demonstrate that the kinase domain of CDK9 directly binds to NF-κB P65 protein, which leads to the CDK9/P65 complex nuclear translocation, P65 phosphorylation, and transcription of inflammatory and hypertrophic genes in cardiomyocytes. This process requires CDK9 Thr-186 phosphorylation and Cyclin T1 presence, but is independent on IKKβ and CDK9-RNAPII pathways. Pharmacological inhibition of CDK9 phosphorylation significantly attenuated Ang II-induced cardiac inflammation, remodeling, and dysfunction in mice. Collectively, Ang II-activated CDK9 directly binds to and phosphorylates P65 to drive cardiac inflammation and remodeling. This study identifies CDK9 as a potential target in heart failure therapeutics |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-15 |
| AnnouncementXML | Submission_2026-06-14_16:11:01.121.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | 仕炬 叶 |
| SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-14 13:00:05 | ID requested | |
| ⏵ 1 | 2026-06-14 16:11:01 | announced | |
Publication List
| Ye S, Zhao Y, Tu H, Han X, Liu T, Gong Y, Lu J, Jin T, Luo W, Qu X, Lai D, Fu G, Liang G, B p65 subunit to drive cardiac inflammation and remodeling. Nat Commun, 17(1):(2026) [pubmed] |
| 10.1038/s41467-026-70410-6; |
Keyword List
| submitter keyword: Angiotensin II |
| CDK9 |
| NF-κB P65 |
| Cardiac remodeling |
| Cardiomyocytes |
Contact List
| Guang Liang |
|---|
| contact affiliation | Hangzhou Medical College |
| contact email | wzmcliangguang@163.com |
| lab head | |
| 仕炬 叶 |
|---|
| contact affiliation | Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University |
| contact email | 3324111@zju.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD074483 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074483
- Label: PRIDE project
- Name: Cardiomyocyte Cyclin-dependent kinase 9 directly binds to and phosphorylates NF-κB p65 subunit to drive cardiac inflammation and remodeling
