PXD074422-1

PXD074422 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide identification of cysteine S-sulfenylation in response to low temperature and low-humidity stress in Dendrobium huoshanense
Description	Objective:Dendrobium huoshanense is a renowned geo-authentic herb, and its excellent quality is closely correlated with the specific combined stress of low temperature and low humidity found in its native habitat.Methods:In this study, we profiled the protein S-sulfenylation landscape of D. huoshanense under simulated low temperature and low humidity stress using Cys-Tag labeling coupled with Data-Independent Acquisition (DIA) mass spectrometry.Results:Physiological analysis revealed a moderate accumulation of H₂O₂ at 12 h of stress, representing an early signaling phase. A total of 9,924 sulfenylation sites mapped to 6,953 proteins were identified. Following stress treatment, 226 sites showed differential modification, 202 of which were upregulated. Motif analysis demonstrated a significant enrichment of lysine and arginine residues flanking the modification sites. Enrichment analysis indicated that S-sulfenylation is primarily involved in vesicle trafficking, carbon metabolism, and amino acid biosynthesis.Conclusion:This study characterizes the response of sulfenylated proteins to low temperature and low humidity stress in D. huoshanense, providing theoretical insights into the molecular mechanisms of plant redox regulation under abiotic stress.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_23:26:23.394.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junjie Guan
SpeciesList	scientific name: Dendrobium huoshanense; NCBI TaxID: NEWT:154293;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-13 02:49:05	ID requested
⏵ 1	2026-03-01 23:26:23	announced

Dataset with its publication pending

submitter keyword: S-sulfenylation, proteomics,Dendrobium huoshanense,H2O2

Shihai Xing
contact affiliation	College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
contact email	xshshihai@163.com
lab head
Junjie Guan
contact affiliation	Anhui University of Chinese Medicine
contact email	1579814154@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074422
     1. Label: PRIDE project
     2. Name: Proteome-wide identification of cysteine S-sulfenylation in response to low temperature and low-humidity stress in Dendrobium huoshanense