PXD074407-1

PXD074407 is an original dataset announced via ProteomeXchange.

Title	DCAF7 regulates hematopoietic stem cell function and differentiation through polycomb repressive complex 1 (PRC1) modulation
Description	DCAF7 is one of the few DCAFs expressed in hematopoietic stem cells (HSCs). Using a conditional knockout mouse model, we discovered that the absence of DCAF7 leads to accumulation of differentiating progenitors in the bone marrow, and impaired self-renewal capacity of HSCs. Furthermore, loss of Dcaf7 accelerates the differentiation of HSCs into the myeloid lineage. At the molecular level, DCAF7 interacts with components of the polycomb repressive complex 1 (PRC1) and promotes their assembly into large macromolecular complexes. Chromatin profiling revealed that loss of DCAF7 leads to increased localization of RING1B at transcriptionally active genomic loci. This increased binding was observed at genes involved in myeloid differentiation and was associated with increased mRNA expression. Lastly, inhibition of KDM2/7 proteins rescues the defective growth of Dcaf7 null HSCs in vitro. Together, these data reveal that DCAF7 is a novel regulator of the PRC1 complex and epigenetic enzymes that control HSC differentiation.
HostingRepository	PRIDE
AnnounceDate	2026-08-20
AnnouncementXML	Submission_2026-08-20_07:55:07.104.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zuo-Fei Yuan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-12 10:32:43	ID requested
⏵ 1	2026-08-20 07:55:07	announced

Dataset with its publication pending

submitter keyword: DCAF7 PRC1 Polycomb RING1B

Anthony A High
contact affiliation	Center for Proteomics & Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA
contact email	anthony.high@stjude.org
lab head
Zuo-Fei Yuan
contact affiliation	St. Jude Children's Research Hospital
contact email	zuo-fei.yuan@stjude.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD074407

PRIDE project URI

• PRIDE
  1. PXD074407
     1. Label: PRIDE project
     2. Name: DCAF7 regulates hematopoietic stem cell function and differentiation through polycomb repressive complex 1 (PRC1) modulation