PXD074405-1

PXD074405 is an original dataset announced via ProteomeXchange.

Title	Hypoxia-Driven Metabolic and Apoptotic Reprogramming Amplifies Immunomodulation in Wharton’s Jelly-Derived Mesenchymal Stem Cells Beyond Bone Marrow Counterparts
Description	Mesenchymal stem cells (MSCs) possess immunomodulatory properties that can be harnessed for treating acute graft-versus-host disease (aGVHD). In this study, we compared bone marrow- (BM) and Wharton’s Jelly-derived (WJ) MSCs and investigated how hypoxia preconditioning (1% O₂, 24 h) influences their immunoregulatory function. Using direct co-cultures with activated peripheral blood mononuclear cells from aGVHD patients, we evaluated T-cell proliferation, Treg induction, macrophage polarization, mitochondrial transfer, and MSC apoptosis. Hypoxia-preconditioned WJ-MSCs (WJ-MSCsHYP) more effectively suppressed T-cell proliferation, enhanced Treg differentiation, promoted M2 macrophage polarization, and improved T-cell metabolic balance via mitochondrial transfer. These effects were primarily driven by apoptosis and occurred independently of efferocytosis. Our findings highlight tissue-specific mechanisms underlying MSCs' immunoregulation and reveal that hypoxia enhances the therapeutic potential of WJ-MSCs. This work provides mechanistic insight into MSCs-based interventions and supports WJ-MSCsHYP as a promising cell source for immunomodulatory therapy in inflammatory disorders such as aGVHD.
HostingRepository	PRIDE
AnnounceDate	2026-02-27
AnnouncementXML	Submission_2026-02-26_23:48:23.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ranjit Sahoo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-12 10:00:30	ID requested
⏵ 1	2026-02-26 23:48:24	announced

Dataset with its publication pending

submitter keyword: Acute graft-versus-host disease, Immunomodulation, Immune-metabolic reprogramming, Hypoxia preconditioning, Apoptosis,Mesenchymal stem cells

Prof. Ranjit Kumar Sahoo
contact affiliation	All India Institute of Medical Sciences, New Delhi, India
contact email	draranjitmd@gmail.com
lab head
Ranjit Sahoo
contact affiliation	All India Institute of Medical Sciences, New Delhi
contact email	drranjitmd@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD074405

PRIDE project URI

• PRIDE
  1. PXD074405
     1. Label: PRIDE project
     2. Name: Hypoxia-Driven Metabolic and Apoptotic Reprogramming Amplifies Immunomodulation in Wharton’s Jelly-Derived Mesenchymal Stem Cells Beyond Bone Marrow Counterparts