PXD074377 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The N’ terminus of Alpha-1 Giardin, a parasitic annexin orthologue, is essential for oligomerization and lipid-binding activity |
| Description | We present a functional analysis of alpha-giardins (αGs), with a particular focus on α1-giardin (α1G), a leading vaccine candidate against giardiasis and a putative virulence-associated UPS substrate. We show that αGs localize to peripheral endocytic compartments (PECs), specialized endo-lysosomal organelles implicated in protein release, and engage in multiprotein complexes enriched in UPS substrates. Using lipid-binding assays, cross-linking mass spectrometry (XL-MS), and structural modelling, we define conserved and divergent features of αG membrane interactions. Focusing on α1G, we combine site-directed mutagenesis, confocal microscopy, and mass photometry to demonstrate a critical role for its short N-terminal region in oligomerization and membrane association. Integration of these data with molecular dynamics simulations reveals how α1G oligomeric state and membrane occupancy are regulated at PEC membranes. Together, our findings establish a mechanistic framework for annexin-mediated UPS in Giardia and provide structural insights relevant to parasite virulence and vaccine development. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-11 |
| AnnouncementXML | Submission_2026-06-11_01:40:31.318.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Manfred Heller |
| SpeciesList | scientific name: Giardia intestinalis assemblage A; NCBI TaxID: NEWT:941442; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-11 23:54:25 | ID requested | |
| ⏵ 1 | 2026-06-11 01:40:31 | announced | |
Publication List
Keyword List
| submitter keyword: lipid, molecular dynamics, annexin A5, unconventional secretion, PIP, oligomerization, endocytic compartments, membrane-binding, Giardia lamblia,Alpha-giardin |
Contact List
| Carmen Faso |
|---|
| contact affiliation | Institute of Cell Biology, University of Bern |
| contact email | carmen.faso@unibe.ch |
| lab head | |
| Manfred Heller |
|---|
| contact affiliation | Proteomics and Mass Spectrometry Core Facility,
Departement for Biomedical Research,
University of Berne |
| contact email | pmscf.dbmr@unibe.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074377
- Label: PRIDE project
- Name: The N’ terminus of Alpha-1 Giardin, a parasitic annexin orthologue, is essential for oligomerization and lipid-binding activity
