PXD074363 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Methylome profiling of SetDB1 deficient ESCs reveals coordinated epigenetic cross-talk during pluripotency |
| Description | SetDB1 is best known as a chromatin modifier catalyzing H3K9me3. However, recent studies show that SetDB1 can mediate H3K27me3-deposition by PRC2, CTCF-binding, recruit DNA methyltransferases, and potentially regulate additional chromatin processes. To gain further insights, we profiled the protein interactome of SetDB1, using a HeLa cell model (BAC clone, PMID 18391959) in which a GFP-tagged SetDB1 is endogenously expressed, closely mimicking the native microenvironment of SetDB1. The GFP tag provides for efficient pulldown using GFP nanobodies fused to agarose beads. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-20 |
| AnnouncementXML | Submission_2026-08-20_06:59:08.702.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Nick Bovee |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | L-methionine removal; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-11 16:55:57 | ID requested | |
| ⏵ 1 | 2026-08-20 06:59:09 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: HeLa, pulldown, epigenetics, co-immunoprecipitation, nuclear extract, GFP, chromatin, SetDB1 |
Contact List
| Hendrik Marks |
|---|
| contact affiliation | Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands |
| contact email | hendrik.marks@ru.nl |
| lab head | |
| Nick Bovee |
|---|
| contact affiliation | RIMLS |
| contact email | n.bovee@outlook.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD074363 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074363
- Label: PRIDE project
- Name: Methylome profiling of SetDB1 deficient ESCs reveals coordinated epigenetic cross-talk during pluripotency
