⮝ Full datasets listing
PXD074358-1
PXD074358 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | 16S ribosomal RNA modification drives transcript-specific translation efficiency |
| Description | Bacterial ribosomal RNAs (rRNAs) are decorated with conserved nucleotide modifications, but the functionality of these modifications is often underexplored. MraW (RsmH) is a 16S rRNA methyltransferase that fine-tunes ribosomal function. We identified a loss-of-function allele in mraW that corrected a late-stage sporulation defect in Bacillus subtilis by bypassing a key sporulation checkpoint via altered translational regulation. Purified ribosomes isolated from delta-mraW cells displayed an approximately 2-fold decrease in translation efficiency; in vivo, delta-mraW cells produced decreased levels of the sporulation checkpoint protein CmpA. This regulation was mediated by sequences from the 5' untranslated region and the coding sequence of cmpA, which form a step-loop structure that occlude early codons of the mRNA. Proteomic analysis revealed that MraW directly or indirectly regulates the production of multiple proteins, some of which form similar structural elements as the cmpA transcript. We propose that MraW modification of 16S rRNA enhances translation efficiency in general, and that specific transcripts, whose gene products are likely required in limiting quantities, have evolved structural features that act as a regulatory mechanism to govern protein levels. This type of regulation may be most apparent in bacteria which exhibit uncoupled transcription and translation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-28 |
| AnnouncementXML | Submission_2026-07-28_11:52:50.671.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lisa Jenkins |
| SpeciesList | scientific name: Bacillus subtilis; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-11 12:26:10 | ID requested | |
| ⏵ 1 | 2026-07-28 11:52:51 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: mraW, sporulation, SpoIVA, DatasetType:Proteomics |
Contact List
| Lisa Jenkins | |
|---|---|
| contact affiliation | NCI, NIH |
| contact email | jenkinsl@mail.nih.gov |
| lab head | |
| Lisa Jenkins | |
| contact affiliation | NCI |
| contact email | jenkinsl@mail.nih.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100810/ |
