PXD074352 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Using magnetic nanoparticles to explore symbiotic interactions |
| Description | All plants and animals form symbiotic associations with microbes, yet many of the underlying mechanisms associated with these interactions remain uncharacterized. There are inherent challenges to studying the cellular and metabolic interactions between eukaryotes and their microbial symbionts, thus new methodologies that enable the discovery of symbiotic processes are continually needed. Here, we explored the use of magnetic nanoparticles (MNPs) as a tool to track aspects of the host innate immune response to symbionts under both ex vivo and in vivo conditions. The symbiotic association between the Hawaiian bobtail squid Euprymna scolopes and its bioluminescent partner Vibrio fischeri was used as a model to explore the potential of MNPs as non-toxic, manipulable agents to investigate aquatic symbiotic associations. Results suggest that host cells can be effectively labeled with MNPs under ex vivo conditions and that the particles can be visualized and tracked within the host animal in vivo using magnetic particle imaging. Proteomic and metabolomic analyses also revealed minimal changes to the host innate immune cells after uptake of MNPs in the presence and absence of V. fischeri. Together, these results suggest that MNPs have minimal biochemical impact on the host cells and can serve as an effective tool to explore aquatic symbiotic interactions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_16:13:54.541.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD074352 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jen Liddle |
| SpeciesList | scientific name: Euprymna scolopes (Hawaiian bobtail squid); NCBI TaxID: NEWT:6613; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-11 10:10:02 | ID requested | |
| ⏵ 1 | 2026-05-24 16:13:55 | announced | |
Publication List
| Guillen Matus DG, Koch EJ, Vijayan N, Good HJ, Samples RM, Rinaldi-Ramos CM, Arnold DP, Nyholm SV, Balunas MJ, Foster JS, Using magnetic nanoparticles to explore symbiotic interactions. Sci Rep, 16(1):(2026) [pubmed] |
| 10.6019/PXD074352; |
| 10.1038/s41598-026-46489-8; |
Keyword List
| submitter keyword: symbiosis |
| Euprymna scolopes, proteomics, metabolomics, hemocytes |
| magnetic nanoparticles |
Contact List
| Jeremy Balsbaugh |
|---|
| contact affiliation | University of Connecticut Proteomics and Metabolomics Facility |
| contact email | jeremy.balsbaugh@uconn.edu |
| lab head | |
| Jen Liddle |
|---|
| contact affiliation | University of Connecticut |
| contact email | jennifer.liddle@uconn.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074352 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074352
- Label: PRIDE project
- Name: Using magnetic nanoparticles to explore symbiotic interactions
