PXD074299-1

PXD074299 is an original dataset announced via ProteomeXchange.

Title	Circadian Rhythms in Cyanobacterial Protein Condensation
Description	Circadian rhythms are endogenously generated daily cycles in physiology that are closely tied to metabolism and can be found in all kingdoms of life. In cyanobacteria, energy storage through glycogen metabolism is highly rhythmic, but the underlying mechanisms remain unclear. Here we find that the glycogen synthase enzyme, GlgA, is rhythmically condensed during growth in constant light. The appearance of condensed foci of GlgA corresponds to inhibition of glycogen synthesis during the morning hours allowing storage to be coordinated with the demand for energy at dusk. Through mutational analysis, we show that the condensation status of GlgA is dictated by the state of the circadian clock rather than the amount of glycogen in the cell. These results reveal a model of circadian control of glycogen metabolism achieved through rhythmic spatial reorganization of a key enzyme.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:13:52.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Rust
SpeciesList	scientific name: Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2); NCBI TaxID: NEWT:1140;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-10 04:13:26	ID requested
⏵ 1	2026-05-24 16:13:53	announced

10.1016/j.isci.2026.115605;

Burton LA, Pattanayak GK, Rust MJ, Rhythmic protein condensation in the circadian regulation of glycogen metabolism. iScience, 29(5):115605(2026) [pubmed]

submitter keyword: Circadian,Cyanobacteria

Michael J Rust
contact affiliation	Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL USA 60637
contact email	mrust@uchicago.edu
lab head
Michael Rust
contact affiliation	University of Chicago
contact email	mrust@uchicago.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074299

PRIDE project URI

• PRIDE
  1. PXD074299
     1. Label: PRIDE project
     2. Name: Circadian Rhythms in Cyanobacterial Protein Condensation