PXD074223-1

PXD074223 is an original dataset announced via ProteomeXchange.

Title	The Extreme Halophilic Archaeon Haloferax volcanii Reduced Fe(III) to Conserve Energy from Extracellular Electron Transfer
Description	Iron availability affects oceanic primary productivity and the strength of the biological carbon pump. However, the mechanism of iron solubility in hypersaline environments is poorly understood. In this work, we investigated the extremely halophilic archaeon Haloferax volcanii to reduce ferrihydrite under 25% salinity, aiming to elucidate its energy metabolism associated with extracellular electron transfer (EET). Ferrihydrite reduction by H. volcanii reached a plateau around the eighth day, with an Fe(III) conversion of approximately 41%. The redox-active substances on the cell surface play a central role via EET. Proteomic analysis revealed that the relative abundance of membrane-bound quinoline b-type cytochrome oxidases (A0A841HB82, A0A841HBL7) increased by 73.8% and 120%, respectively, when H. volcanii used ferrihydrite rather than O2 as the terminal electron acceptor. Moreover, H. volcanii was found to secrete riboflavin which can function as an electron shuttle mediating indirect EET. Our findings provide insights into the survival and evolutionary strategies of early life in anaerobic environments on Earth , and the EET capability of H. volcanii highlights its potential applicability in the treatment of pollutants in hypersaline wastewater.
HostingRepository	iProX
AnnounceDate	2026-02-08
AnnouncementXML	Submission_2026-02-08_23:24:58.822.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yijing Miao
SpeciesList	scientific name: Haloferax volcanii; NCBI TaxID: 2246;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-08 23:23:58	ID requested
⏵ 1	2026-02-08 23:24:59	announced

Dataset with its publication pending

submitter keyword: Archaea, Extracellular electron transfer, Hypersaline, Dissimilatory Fe(III) reduction, Biogeochemical cycle of iron

Feng Zhao
contact affiliation	Institute of Urban Environment, Chinese Academy of Science
contact email	fzhao@iue.ac.cn
lab head
Yijing Miao
contact affiliation	Institute of Urban Environment, Chinese Academy of Sciences
contact email	yijing_miao@163.com
dataset submitter

iProX dataset URI