PXD074081-1

PXD074081 is an original dataset announced via ProteomeXchange.

Title	Proteomic and Phenotypic Profiling of Replicative-Senescent HFF-1 Fibroblasts Under Brief Heat Shock
Description	As the largest human organ, the skin experiences lifelong exposure to intrinsic/extrinsic factors that over time diminish its functional capacity and structural integrity. Skin aging involves cellular dysfunction and the loss or fragmentation of extracellular matrix (ECM) fibers, clinically presenting as wrinkles, slackening, and pigmentary abnormalities. The heat shock response (HSR) is a gene regulatory program that controls the expression of molecular chaperones associated with aging, cancer, and neurodegenerative disorders. By maintaining cellular homeostasis and facilitating DNA repair, HSR exerts protective effects against skin aging, as utilized in aesthetic technologies such as radiofrequency and focused ultrasound. This study aimed to investigate the mechanism, optimal conditions, and potential risks of short-term heat shock (HS) on the senescence process of human foreskin fibroblasts (HFF-1), providing experimental evidence to support the application of thermal stress in delaying skin aging. A replicative senescence model of HFF-1 cells was first established. Subsequently, cells were subjected to HS at 41 °C, 45 °C, and 49 °C, with a control group maintained at 37 °C. Assessments, including cell proliferation and viability assays, apoptosis analysis, reactive oxygen species (ROS) levels, Western blot, and heat shock proteins (HSPs) mRNA expression, demonstrated that 49 °C HS induced irreversible cellular damage. In contrast, 30 min HS at 41 °C and 45 °C attenuated senescence-associated phenotypes to varying extents under our experimental conditions.
HostingRepository	iProX
AnnounceDate	2026-02-05
AnnouncementXML	Submission_2026-02-05_00:39:29.579.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-05 00:39:09	ID requested
⏵ 1	2026-02-05 00:39:30	announced

Dataset with its publication pending

submitter keyword: skin aging, heat shock response, replicative senescence, molecular chaperones, extracellular matrix remodeling

Lei Liu
contact affiliation	Beijing Technology and Business University
contact email	liulei@btbu.edu.cn
lab head
Yue Yang
contact affiliation	Beijing Technology and Business University
contact email	m15120011938@163.com
dataset submitter

iProX dataset URI