PXD074053-1

PXD074053 is an original dataset announced via ProteomeXchange.

Title	mTRACE: Modification-enabled Tracking of RNA-associated Cellular Events
Description	The life cycle of RNA is orchestrated by a series of molecular events whose dynamic regulation is central to gene expression and cellular function. However, existing methods face limitations in continuously recording these events in living cells. Here we introduce mTRACE (Modification-enabled Tracking of RNA-associated Cellular Events), a molecular recording framework that leverages RNA modification enzymes to capture the history of RNA-associated cellular processes. By systematically screening a pool of 34 candidate enzymes, we identified N6-adenosine methyltransferase M.EcoGII as a highly efficient and unbiased modifier. mTRACE exploits M.EcoGII to introduce natural and non-disruptive RNA modification, writing durable marks on transcripts undergoing specific molecular events. We demonstrate that mTRACE enables precise mapping of RNA-protein interactions, including tracking and retrospective detection of ALYREF binding; quantification of both translational activity and efficiency by coupling M.EcoGII to ribosomal proteins; and spatial recording of RNA residence across subcellular compartments using engineered M.EcoGII variants. mTRACE provides a powerful solution for dissecting RNA dynamic regulation and opens a new avenue for multi-dimensional recording of RNA biology with spatiotemporal resolution.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_07:15:07.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wu Fu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-04 05:34:13	ID requested
⏵ 1	2026-09-09 07:15:08	announced

Dataset with its publication pending

submitter keyword: human

Fu Wu
contact affiliation	Sun Yat-sen university, China
contact email	wufu@mail2.sysu.edu.cn
lab head
Wu Fu
contact affiliation	Sun Yat-sen university
contact email	wufu@mail2.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD074053

PRIDE project URI

• PRIDE
  1. PXD074053
     1. Label: PRIDE project
     2. Name: mTRACE: Modification-enabled Tracking of RNA-associated Cellular Events