PXD074021-1

PXD074021 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of RNA-binding proteins interacting with tRF-1432 in breast cancer
Description	The purpose of this proteomic study was to identify RNA-binding proteins that specifically interact with the tRNA-derived fragment tRF-1432 in breast cancer cells. tRF-1432, a 5′-tRNA-derived stress-induced RNA generated from tRNA^Val-CAC-2-1, has been implicated in post-transcriptional regulation through its potential binding to RNA-binding proteins. To explore this mechanism, biotin-labeled tRF-1432 probes were used in RNA pulldown assays performed in MCF-7 and MDA-MB-231 breast cancer cell lines. The eluates were subjected to LC-MS/MS analysis, enabling the identification of candidate proteins potentially associated with tRF-1432.
HostingRepository	iProX
AnnounceDate	2026-02-03
AnnouncementXML	Submission_2026-08-31_20:13:54.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhan Dai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-03 18:38:53	ID requested
⏵ 1	2026-08-31 20:13:55	announced

Dataset with its publication pending

submitter keyword: tRF-1432, RNA-binding proteins, breast cancer, RNA pulldown, LC-MS/MS, chemoresistance

Yangyang Cui
contact affiliation	The First Affiliated Hospital with Nanjing Medical University
contact email	cuiyy@njmu.edu.cn
lab head
Yuhan Dai
contact affiliation	Women’s Hospital of Nanjing Medical University & Nanjing Women and Children's Healthcare Hospital
contact email	daiyuhan0210@163.com
dataset submitter

iProX dataset URI