PXD074013 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A global S-palmitoylation map of Trypanosoma cruzi identifies ABCA10 as a key component of a redundant heme-acquisition system |
| Description | The survival and continued transmission of the human infecting parasite Trypanosoma cruzi depends on the dynamic remodeling of its membrane proteome, yet the post-translational networks governing this plasticity remain largely obscure. Here, we deployed metabolic labeling coupled with quantitative mass spectrometry to generate the first comprehensive S-palmitoylome of T. cruzi. We defined a high-confidence atlas of 254 S-palmitoylated proteins, revealing an enrichment in signaling factors, cytoskeletal regulators, and membrane transporters. Leveraging our aptazyme-based SHARK conditional knockdown system for scalable genetic screening, we validated palmitoylated candidates and uncovered a critical link between lipid signaling and nutrient acquisition. Our functional screen identified ABCA10, a previously uncharacterized ATP-binding cassette (ABC)-transporter, as central to heme homeostasis. Strikingly, depletion of ABCA10 triggered a massive compensatory surge in cytostome-mediated endocytosis, rendering this normally dispensable pathway conditionally essential for survival. We further demonstrate that ABCA10 is a heme-responsive transporter that dynamically redistributes from the flagellar pocket to lysosomal compartments upon heme starvation, exposing a redundant, multi-modal strategy for scavenging this essential cofactor. This study not only provides a foundational proteomic resource but also exposes a novel, druggable vulnerability at the intersection of lipid signaling and metabolic survival. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-30 |
| AnnouncementXML | Submission_2026-07-30_11:57:23.196.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD074013 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sophe Alvarez |
| SpeciesList | scientific name: Trypanosoma cruzi; NCBI TaxID: NEWT:5693; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-03 16:31:33 | ID requested | |
| ⏵ 1 | 2026-07-30 11:57:23 | announced | |
Publication List
Keyword List
| submitter keyword: Eclipse orbitrap, S-palmitoylome, membrane proteome,Trypanosoma cruzi |
Contact List
| Sophie Alvarez |
|---|
| contact affiliation | Proteomics and Metabolomics Facility, University of Nebraska - Lincoln |
| contact email | salvarez@unl.edu |
| lab head | |
| Sophe Alvarez |
|---|
| contact affiliation | University of Nebraska - Lincoln |
| contact email | salvarez@unl.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074013
- Label: PRIDE project
- Name: A global S-palmitoylation map of Trypanosoma cruzi identifies ABCA10 as a key component of a redundant heme-acquisition system
