PXD074001-1

PXD074001 is an original dataset announced via ProteomeXchange.

Title	Rat skin wound healing treated with SS or SF
Description	Rapid and functional skin wound healing remains a major clinical challenge, yet current biomaterial-based therapies largely function as passive scaffolds or delivery vehicles. Despite their widespread clinical use, whether biomaterials possess intrinsic bioactivities capable of actively instructing endogenous regenerative programs remains poorly understood. Here, independent of bulk structural or mechanical cues and exogenous biomolecules, we demonstrate that silk fibroin (SF), a clinically approved biomaterial, intrinsically programs keratinocytes into a Ccl27⁺ migratory-paracrine pro-regenerative state that coordinates epidermal-dermal crosstalk to drive regenerative wound healing. Using integrated scRNA-seq, proteomics, and keratinocyte-specific genetic perturbation, we show that SF engages the integrin receptor ITGA2 on keratinocytes to activate the PI3K-Akt pathway, relieving p53-mediated repression of Ccl27. Elevated Ccl27 endows keratinocytes with enhanced migratory capacity to facilitate re-epithelialization, while Ccl27 secreted from SF-activated keratinocytes simultaneously acts in a paracrine manner to promote fibroblast proliferation and dermal remodeling, collectively enabling efficient wound closure and appendage reconstruction. Keratinocyte-specific conditional knockdown of Ccl27 largely abrogates the pro-regenerative effects of SF in vivo. SF elicits only minimal immune activation during wound healing, preserving an immunocompatible regenerative microenvironment. These findings uncover an intrinsic bioactivity of SF that actively programs a pro-regenerative keratinocyte state, establishing a paradigm in which biomaterials function as instructive cues rather than passive scaffolds to drive functional tissue repair.
HostingRepository	PRIDE
AnnounceDate	2026-09-04
AnnouncementXML	Submission_2026-09-03_19:43:33.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haoyang Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-03 13:30:48	ID requested
⏵ 1	2026-09-03 19:43:33	announced

Dataset with its publication pending

submitter keyword: None

Wei Zhang
contact affiliation	School of Medicine, Southeast University, 210009, Nanjing, China.
contact email	zhang.wei@seu.edu.cn
lab head
Haoyang Liu
contact affiliation	Southeast University
contact email	riuhaoyang@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD074001

PRIDE project URI

• PRIDE
  1. PXD074001
     1. Label: PRIDE project
     2. Name: Rat skin wound healing treated with SS or SF