PXD074000-1

PXD074000 is an original dataset announced via ProteomeXchange.

Title	Fractionated proteomics identifies a protein network mitigating resistance exercise-induced damage in human skeletal muscle
Description	Resistance exercise (RE) improves strength and muscle mass, with multiple benefits for human health. However, intense RE also induces acute myofibrillar damage. The molecular mechanisms that preserve, mark, degrade and restore damaged proteins in order to to keep skeletal muscle working under RE are incompletely understood. Here we study acute molecular and structural responses to RE-induced damage in repeatedly sampled human skeletal muscle in the unadapted, adapted and deadapted state. We show that acute, repeated and interrupted RE induces dynamic changes of the protein landscape associated with the muscular cytoskeleton and myofibrillar lesions. These changes correlate with changes in phosphorylation that are part of a larger molecular signaling footprint indicative of adaptation and deadaptation. We further identify a functional protein network linked to the muscle maintenance protein BAG3, which comprises the mechanosensory proteins PDLIM3 and XIRP1, the small heat shock proteins HSPB1 and HSPB5 and the lipid droplet associated protein PLIN5. All network components exhibit altered phosphorylation and increased cytoskeletal association after damaging RE and cooperate in the recognition of strained muscular structures and the degradation of damaged proteins through chaperone-assisted selective autophagy (CASA). Our study thus reveals previously unrecognized key regulators of muscle homeostasis in humans.
HostingRepository	PRIDE
AnnounceDate	2026-05-04
AnnouncementXML	Submission_2026-05-04_06:45:27.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Bonini
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-03 13:16:09	ID requested
⏵ 1	2026-05-04 06:45:27	announced

Dataset with its publication pending

submitter keyword: None

Dominic Winter
contact affiliation	Research Center One Health Ruhr, University Alliance Ruhr & University Hospital Essen, Medical Faculty, University Duisburg-Essen
contact email	Dominic.Winter@uk-essen.de
lab head
Sara Bonini
contact affiliation	Institute of Biochemistry and Molecular Biology
contact email	sarab@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074000
     1. Label: PRIDE project
     2. Name: Fractionated proteomics identifies a protein network mitigating resistance exercise-induced damage in human skeletal muscle