PXD073978-1

PXD073978 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAcylation of intrinsically disordered regions selectively tunes RBP phase separation and function
Description	Intrinsically disordered regions (IDRs) are central to liquid–liquid phase separation (LLPS), yet how post-translational modifications (PTMs) selectively regulate IDR-driven phase behavior remains unclear. Here, we systematically analyze PTMs across RNA-binding proteins (RBPs), a protein class highly enriched in IDRs. By integrating curated PTM databases with quantitative chemoenzymatic profiling, we find that although many RBPs are O-GlcNAc modified, only a small fraction (~5%) exhibits high-level O-GlcNAcylation. Notably, all highly modified RBPs undergo LLPS but display diverse condensate dynamics, indicating context-dependent regulation. Mechanistic analyses of representative candidates reveal that O-GlcNAcylation can exert opposing effects on LLPS depending on protein context.
HostingRepository	iProX
AnnounceDate	2026-02-03
AnnouncementXML	Submission_2026-02-02_22:44:03.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lihui Lao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-02 22:43:43	ID requested
⏵ 1	2026-02-02 22:44:04	announced

Dataset with its publication pending

submitter keyword: O-GlcNAcylation, IDR, RBP

Shixian Lin
contact affiliation	Zhejiang University
contact email	sxlin@zju.edu.cn
lab head
Lihui Lao
contact affiliation	Zhejiang University
contact email	lihuilao@zju.edu.cn
dataset submitter

iProX dataset URI