PXD073977 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in Murine Asthma Model |
| Description | Extracellular vesicles (EVs) are increasingly recognized as key mediators of intercellular communication and immune regulation. However, their proteomic composition in allergic asthma remains poorly understood. In this study, we performed tandem mass tag (TMT)-based quantitative proteomic analyses of the whole bronchoalveolar lavage fluid (BALF) supernatant and EVs enriched from BALF in a murine model of house dust mite (HDM)-induced asthma. Using LC-MS/MS analysis in the data-dependent acquisition (DDA) mode, we identified more than 400 proteins across all samples, revealing distinct enrichment of canonical EV markers in EV fractions. HDM challenge induced significant upregulation of Th2-associated proteins, e.g., CLCA1, FCGBP, CHIL3, CHIL4, and RETNLA, consistent with hallmark features of asthma. Notably, proteins such as EPX and CKM were detected exclusively in EVs, not in whole BALF, underscoring the potential of EVs to selectively carry disease-relevant proteins. Our results provided insights into the distinct proteomic profiles of EV-associated versus BALF proteins, highlighting potential EV-mediated mechanisms in asthma pathogenesis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-20 |
| AnnouncementXML | Submission_2026-04-20_13:20:27.948.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zachary Hutchins |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-02 21:30:16 | ID requested | |
| ⏵ 1 | 2026-04-20 13:20:28 | announced | |
Publication List
Keyword List
| submitter keyword: ultracentrifugation, mouse model, asthma, TMT, mass spectrometry,Extracellular vesicles, HDM, bronchoalveolar lavage fluid |
Contact List
| Yinsheng Wang |
|---|
| contact affiliation | Department of Chemistry, University of California, Riverside, CA 92502, USA |
| contact email | yinsheng@ucr.edu |
| lab head | |
| Zachary Hutchins |
|---|
| contact affiliation | University of California, Riverside |
| contact email | zachary.hutchins@email.ucr.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD073977 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073977
- Label: PRIDE project
- Name: Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in Murine Asthma Model
