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PXD073964-2
PXD073964 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An Orthogonal Extraction Workflow for High-Depth Plasma Proteomics with Metabolomic Compatibility from Low Microliter-Scale Samples |
| Description | Herein, we report an orthogonal extraction workflow that achieves enhanced proteomic profiling depth while preserving metabolomic compatibility from a mere 10 µL of plasma. We demonstrate that a ternary solvent mixture (TSM) of methanol, acetonitrile, and acetone exhibits distinct chemical orthogonality to perchloric acid (PCA) precipitation, with each strategy recovering reproducible yet partially non-overlapping subsets of plasma proteins. By combining orthogonal extraction with PCA and TSM to deplete high-abundant proteins, we expanded plasma proteome coverage to 1136 proteins—representing a 49% increase compared to PCA alone and a 2.6-fold enhancement relative to the standard neat plasma processing protocol. This marked improvement in low-abundance protein detection enabled efficient coverage of 101 FDA-approved circulating biomarkers. For metabolomics, the workflow retained comprehensive coverage (1174 metabolites) comparable to standard methanol extraction, with superior qualitative reproducibility and quantitative consistency across technical replicates. This workflow may enable protein-centric biomarker discovery augmented by orthogonal metabolic insights from microliter-scale samples, providing a transformative tool to surmount the limitations of standalone proteomics and accelerate the clinical translation of candidate biomarkers. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-03 |
| AnnouncementXML | Submission_2026-07-08_02:25:39.221.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chunping Zhao |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-02 19:11:56 | ID requested | |
| 1 | 2026-07-01 00:50:23 | announced | |
| ⏵ 2 | 2026-07-08 02:25:40 | announced | 2026-07-08: Add info |
Publication List
| Zhao C, Hu H, Qian X, Zhang W, Qin W, An orthogonal extraction workflow for high-depth plasma proteomics with metabolomic compatibility from low microliter-scale samples. Anal Methods, 18(15):3074-3085(2026) [pubmed] |
Keyword List
| submitter keyword: Proteomics, Enrichment, Mass spectrometry, Plasma, Sample preparation |
Contact List
| Weijie Qin | |
|---|---|
| contact affiliation | State Key Laboratory of Medical Proteomics, National Center for Protein Sciences (Beijing), Academy of Military Medical Sciences |
| contact email | aunp_dna@126.com |
| lab head | |
| Chunping Zhao | |
| contact affiliation | State Key Laboratory of Medical Proteomics, National Center for Protein Sciences (Beijing), Academy of Military Medical Sciences |
| contact email | zhaochuanping513@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
