PXD073946 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion |
| Description | Nuclear pore complexes (NPCs) enable nucleocytoplasmic transport. While NPCs primarily localize to the nuclear envelope (NE), they also appear in cytoplasmic endoplasmic reticulum (ER) membranes called annulate lamellae (AL). Though discovered in the mid-20th century, AL’s function and biogenesis remain unclear. Previously considered exclusive to embryonic and malignant cells, we find AL in somatic mammalian cells. Under normal conditions, AL store pre-assembled NPCs (AL-NPCs) that integrate into the NE during G1 to support nuclear expansion. Upon pathological stimuli, AL transfer to the NE is impaired, leading to their cytoplasmic accumulation. RanBP2 (Nup358) is essential for AL biogenesis, with its phenylalanine-glycine repeats promoting AL-NPC scaffold oligomerization. ER-associated Climp63 (CKAP4) directs AL-NPCs to ER sheets and the NE. This AL-driven nuclear pore formation is complementary to the canonical routes, constituting a distinct NPC assembly pathway. Our work uncovers the biogenesis mechanism of AL and the nuclear function of this key cellular organelle. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_16:13:42.875.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Guillaume CHEVREUX |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-02 14:38:10 | ID requested | |
| ⏵ 1 | 2026-05-24 16:13:43 | announced | |
Publication List
| 10.1038/s41467-026-71101-y; |
| Lin J, Agote-Aran A, Liao Y, Cloarec M, Andronov L, Schoch RL, Ronchi P, Cochard V, Zhu R, Grandgirard E, Liu X, Lem, é, e MV, Kleiss C, Golzio C, Ruff M, Chevreux G, Schwab Y, Klaholz BP, Sumara I, RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion. Nat Commun, 17(1):(2026) [pubmed] |
Keyword List
| submitter keyword: nuclear expansion, Climp63 (CKAP4), G1, single molecule localization microscopy (SMLM),annulate lamellae, nuclear growth, FG repeats, RanBP2 (Nup358), nuclear pore complex, cytoplasmic nucleoporin foci |
Contact List
| Izabela Sumara |
|---|
| contact affiliation | Izabela Sumara, Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France |
| contact email | sumara@igbmc.fr |
| lab head | |
| Guillaume CHEVREUX |
|---|
| contact affiliation | Institut Jacques Monod, CNRS UMR7592 |
| contact email | guillaume.chevreux@ijm.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073946
- Label: PRIDE project
- Name: RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion
