PXD073932 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphorylation of the transcriptional-corepressor SPEN in naïve mouse embryonic stem cells and under artificial induction of X chromosome inactivation |
| Description | We developed a CRISPR/Cas9-mediated base-editing screen to functionally screen endogenous proteins. We screened SPEN, a key factor in X chromosome inactivation, for loss-of-function mutations. Some of the screen hits were mutations of serine residues that could be potential phosphorylation sites (by prediction) and could serve as regulatory sites for the function and/or structure of SPEN. To follow up on our observations, we analyzed phosphorylation sites of SPEN in mouse embryonic stem cells, in which both X chromosomes are active (“noDox” sample), and in cells in which X chromosome inactivation was artificially induced by Xist expression (“Dox” sample). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-08 |
| AnnouncementXML | Submission_2026-04-08_04:15:48.510.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Corinne Kaufmann |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | phosphorylated residue; acetylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-02 08:42:35 | ID requested | |
| ⏵ 1 | 2026-04-08 04:15:49 | announced | |
Publication List
Keyword List
| submitter keyword: SPEN, XCI, dosage compensation, X chromosome, IDR, gene regulation, timsTOF, phosphoproteomics, Xist |
Contact List
| Prof. Dr. Anton Wutz |
|---|
| contact affiliation | Institute of Molecular Health Sciences, Department of Biology, Swiss Federal Institute of Technology ETH, Zurich |
| contact email | awutz@ethz.ch |
| lab head | |
| Corinne Kaufmann |
|---|
| contact affiliation | ETH Zurich
Institute of Molecular Health Sciences |
| contact email | corinne.kaufmann@biol.ethz.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073932
- Label: PRIDE project
- Name: Phosphorylation of the transcriptional-corepressor SPEN in naïve mouse embryonic stem cells and under artificial induction of X chromosome inactivation
