PXD073906-1

PXD073906 is an original dataset announced via ProteomeXchange.

Title	SRSF1 sustains the nucleolus by charge-modulated pH regulation
Description	Nucleolus is a multiphase biomolecular assembly containing three distinct subcompartments. Evidence is just emerging that nucleolar proteins with characteristic electrochemical properties condense to generate a pH gradient, which serves as a key driving force for organizing the nucleolar sub-phases. Given the indispensable functionality of the nucleolus in most, if not all, cellular activities, it is vital for a cell to sense potential fluctuations in the nucleolar pH and subsequently maintain pH homeostasis. The mechanisms by which a cell achieves this task remain poorly decoded. Here, we show that splicing factor SRSF1 is shuttled from nuclear speckle (NS) to the nucleolus during times of stress, which interrogates the nucleolar pH. SRSF1 nucleolar localization is reliant on an acidic patch. Loss of SRSF1 impedes pH homeostasis in the dark nucleolar cap and subsequently obstructs the restoration of the nucleolar multiphase and function. The RS (arginine/serine rich) domain of SRSF1, due to its high isoelectric point (pI), directly alkalinizes the nucleolar microenvironment. Interestingly, synthetic arginine-rich dipeptide derivatives of SRSF1 RS domain safeguard the nucleolus from pH and functional disturbance. Our findings uncover unprecedented mechanistic insights into nucleolar pH-sensing and regulation.
HostingRepository	iProX
AnnounceDate	2026-01-31
AnnouncementXML	Submission_2026-07-08_02:35:12.154.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suibin Ma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-01 19:16:08	ID requested
⏵ 1	2026-07-08 02:35:12	announced

Dataset with its publication pending

submitter keyword: Nucleolus, Dark nucleolar caps, SRSF1, pH, Alkaline

Bo Wang
contact affiliation	School of Life Sciences, Xiamen University
contact email	bowang@xmu.edu.cn
lab head
Suibin Ma
contact affiliation	School of Life Sciences, Xiamen University
contact email	masuibin11@163.com
dataset submitter

iProX dataset URI