PXD073897-1

PXD073897 is an original dataset announced via ProteomeXchange.

Title	Evaluating the Molecular Effects of Two Quinoline-Containing Anti-Proliferative Compounds Using a Multi-Omics Approach
Description	Cancer is a major health concern worldwide, necessitating the discovery of more targeted approaches adequate management and therapy of tumors that are especially malignant, such as triple-negative breast cancer (TNBC). Through various biochemical studies carried out on TNBC, the MAPK pathway was implicated in cancer progression. MAPK involves the activation of multiple kinases, one of which is the C-RAF kinase. Therefore, small-molecule kinase inhibitors that can inhibit C-RAF and the MAPK pathway are of great interest in cancer research. This study investigates two C-RAF kinase inhibitors synthesized as quinoline-containing small molecules based on the design of the established kinase inhibitor sorafenib and other lead compounds. MDA-MB-468 and BT-549, both TNBC cell lines, were treated with the compounds separately and in combination, followed by metabolomics and proteomics analyses using LC-MS/MS. Upon identifying altered metabolic pathways and dysregulated proteins, the compounds’ mechanisms of action were found to involve DNA damage and repair, tRNA synthesis, protein expression, and energy production. The combination of the two C-RAF kinase inhibitors on TNBC cell lines led to earlier impact on amino acid metabolism and subsequent protein expression. This study hopes to enrich the current understanding of cancer biochemistry and reveal key biomarkers which can ultimately be targeted cancer therapies.
HostingRepository	PRIDE
AnnounceDate	2026-04-12
AnnouncementXML	Submission_2026-04-12_09:30:48.526.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Core Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-01 17:08:55	ID requested
⏵ 1	2026-04-12 09:30:49	announced

Dataset with its publication pending

submitter keyword: Triple-negative breast cancer

Kinase inhibitors

Proteomics

Metabolomics

LC-MS/MS-QTOF

Prof. Mohammad Semreen
contact affiliation	University of Sharjah
contact email	msemreen@sharjah.ac.ae
lab head
Core Facility
contact affiliation	Sharjah Institute for Medical Research, University of Sharjah
contact email	tims-tof@sharjah.ac.ae
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073897
     1. Label: PRIDE project
     2. Name: Evaluating the Molecular Effects of Two Quinoline-Containing Anti-Proliferative Compounds Using a Multi-Omics Approach